Skip to Main content Skip to Navigation
Journal articles

Differentiation in vivo of cardiac committed human embryonic stem cells in postmyocardial infarcted rats.: Human ES cells in postmyocardial infarction

Abstract : Human embryonic stem (HES) cells can give rise to cardiomyocytes in vitro. However, whether undifferentiated HES cells also feature a myocardial regenerative capacity after in vivo engraftment has not been established yet. We compared two HES cell lines (HUES-1 and I6) that were specified toward a cardiac lineage by exposure to bone morphogenetic protein-2 (BMP2) and SU5402, a fibroblast growth factor receptor inhibitor. Real-time polymerase chain reaction (PCR) revealed that the cardiogenic inductive factor turned on expression of mesodermal and cardiac genes (Tbx6, Isl1, FoxH1, Nkx2.5, Mef2c, and alpha-actin). Thirty immunosuppressed rats underwent coronary artery ligation and, 2 weeks later, were randomized and received in-scar injections of either culture medium (controls) or BMP2 (+/-SU5402)-treated HES cells. After 2 months, human cells were detected by anti-human lamin immunostaining, and their cardiomyocytic differentiation was evidenced by their expression of cardiac markers by reverse transcription-PCR and immunofluorescence using an anti-beta myosin antibody. No teratoma was observed in hearts or any other organ of the body. The ability of cardiac-specified HES cells to differentiate along the cardiomyogenic pathway following transplantation into infarcted myocardium raises the hope that these cells might become effective candidates for myocardial regeneration.
Complete list of metadatas

Cited literature [32 references]  Display  Hide  Download

https://www.hal.inserm.fr/inserm-00149211
Contributor : Michel Puceat <>
Submitted on : Monday, August 17, 2009 - 2:32:29 PM
Last modification on : Wednesday, August 19, 2020 - 11:16:51 AM
Long-term archiving on: : Thursday, April 8, 2010 - 5:46:15 PM

Identifiers

Collections

Citation

André Tomescot, Julia Leschik, Valérie Bellamy, Gilbert Dubois, Emmanuel Messas, et al.. Differentiation in vivo of cardiac committed human embryonic stem cells in postmyocardial infarcted rats.: Human ES cells in postmyocardial infarction. Stem Cells / Stem Cells (Miamisburg), Alphamed Press, 2007, 25 (9), pp.2200-5. ⟨10.1634/stemcells.2007-0133⟩. ⟨inserm-00149211⟩

Share

Metrics

Record views

729

Files downloads

1362