Effect of polyunsaturated fatty acids deficiency on oxidative phosphorylation in rat liver mitochondria.

Eric Fontaine 1 Marguerite Moussa 2 Anne Devin 3 Jésus Garcia 2 Jacques Ghisolfi 4 Michel Rigoulet 3 Xavier Leverve 1
1 Bioénergétique fondamentale et appliquée
2 Laboratoire de Biochimie [Purpan]
3 IBGC - Institut de biochimie et génétique cellulaires
4 Département de Pédiatrie
Abstract : Liver mitochondria isolated from controls or polyunsaturated fatty acid (PUFA) deficient rats were studied for oxidative phosphorylation. A PUFA-deficient diet led to a dramatic change in the fatty acid composition of mitochondrial lipid content, similar to that reported in the literature. Besides the changes in lipid composition, mitochondrial volume was enlarged (+45% in state 4 and two-fold in state 3). State 4 respiration was increased together with a decrease in protonmotive force. The non-ohmicity of the relationship between non-phosphorylating respiration and protonmotive force was more pronounced in the PUFA-deficient group. State 3 oxygen consumption as well as the rate of ATP synthesis showed no difference between the two groups, whereas the protonmotive force decreased substantially in mitochondria from PUFA-deficient animals. In contrast, ATP/O ratios were decreased in the PUFA-deficient group when determined at subsaturating ADP concentration. Taken together, these results are in agreement with both an increased non-ohmic proton leak and an increased redox slipping. The relative importance of these two effects on the overall efficiency of oxidative phosphorylation depends on both the rate of oxidative phosphorylation and the maintained protonmotive force. Hence, in isolated mitochondria the respective role of each effect may vary between state 4 and state 3.
Type de document :
Article dans une revue
Biochimica et Biophysica Acta - Molecular Cell Research, Elsevier, 1996, 1276 (3), pp.181-7
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http://www.hal.inserm.fr/inserm-00390243
Contributeur : Sarah Hamant <>
Soumis le : lundi 1 juin 2009 - 12:49:21
Dernière modification le : mardi 11 septembre 2018 - 15:18:57

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  • HAL Id : inserm-00390243, version 1
  • PUBMED : 8856103

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Eric Fontaine, Marguerite Moussa, Anne Devin, Jésus Garcia, Jacques Ghisolfi, et al.. Effect of polyunsaturated fatty acids deficiency on oxidative phosphorylation in rat liver mitochondria.. Biochimica et Biophysica Acta - Molecular Cell Research, Elsevier, 1996, 1276 (3), pp.181-7. 〈inserm-00390243〉

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