Effect of polyunsaturated fatty acids deficiency on oxidative phosphorylation in rat liver mitochondria. - Inserm - Institut national de la santé et de la recherche médicale Accéder directement au contenu
Article Dans Une Revue Biochimica et Biophysica Acta - Molecular Cell Research Année : 1996

Effect of polyunsaturated fatty acids deficiency on oxidative phosphorylation in rat liver mitochondria.

Résumé

Liver mitochondria isolated from controls or polyunsaturated fatty acid (PUFA) deficient rats were studied for oxidative phosphorylation. A PUFA-deficient diet led to a dramatic change in the fatty acid composition of mitochondrial lipid content, similar to that reported in the literature. Besides the changes in lipid composition, mitochondrial volume was enlarged (+45% in state 4 and two-fold in state 3). State 4 respiration was increased together with a decrease in protonmotive force. The non-ohmicity of the relationship between non-phosphorylating respiration and protonmotive force was more pronounced in the PUFA-deficient group. State 3 oxygen consumption as well as the rate of ATP synthesis showed no difference between the two groups, whereas the protonmotive force decreased substantially in mitochondria from PUFA-deficient animals. In contrast, ATP/O ratios were decreased in the PUFA-deficient group when determined at subsaturating ADP concentration. Taken together, these results are in agreement with both an increased non-ohmic proton leak and an increased redox slipping. The relative importance of these two effects on the overall efficiency of oxidative phosphorylation depends on both the rate of oxidative phosphorylation and the maintained protonmotive force. Hence, in isolated mitochondria the respective role of each effect may vary between state 4 and state 3.
Fichier non déposé

Dates et versions

inserm-00390243 , version 1 (01-06-2009)

Identifiants

  • HAL Id : inserm-00390243 , version 1
  • PUBMED : 8856103

Citer

Eric M. Fontaine, Marguerite Moussa, Anne Devin, Jésus Garcia, Jacques Ghisolfi, et al.. Effect of polyunsaturated fatty acids deficiency on oxidative phosphorylation in rat liver mitochondria.. Biochimica et Biophysica Acta - Molecular Cell Research, 1996, 1276 (3), pp.181-7. ⟨inserm-00390243⟩

Collections

INSERM UGA CNRS
288 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More