Antibodies for CFTR studies.
Filipa Mendes
(1)
,
Carlos M. Farinha
(1, 2)
,
Mónica Roxo-Rosa
(1, 2)
,
Pascale Fanen
(3)
,
Aleksander Edelman
(4)
,
Robert Dormer
(5)
,
Margaret Mcpherson
(5)
,
Heather Davidson
(6)
,
Edith Puchelle
(7)
,
Hugo R. de Jonge
(8)
,
Ghanshyam D. Heda
(9)
,
Martina Gentzsch
(10)
,
Gergely Lukacs
(11)
,
Deborah Penque
(1)
,
Margarida D. Amaral
(1, 2)
1
CHG -
Center of Human Genetics
2 DCB - Department of Chemistry and Biochemistry
3 Génétique moléculaire et physiopathologie
4 UMR_S467 - Régulation des systèmes de transport dans les épithéliums
5 DMBI - Department of Medical Biochemistry and Immunology
6 DMS - Department of Medical Sciences
7 Dynamique cellulaire et moléculaire de la muqueuse respiratoire
8 DB - Department of Biochemistry [Netherlands]
9 VAMCDM - The Veterans Affairs Medical Center and The Department of Medecine
10 MCS - Mayo Clinic Scottsdale
11 HSCRI - Hospital for Sick Children Research Institute
2 DCB - Department of Chemistry and Biochemistry
3 Génétique moléculaire et physiopathologie
4 UMR_S467 - Régulation des systèmes de transport dans les épithéliums
5 DMBI - Department of Medical Biochemistry and Immunology
6 DMS - Department of Medical Sciences
7 Dynamique cellulaire et moléculaire de la muqueuse respiratoire
8 DB - Department of Biochemistry [Netherlands]
9 VAMCDM - The Veterans Affairs Medical Center and The Department of Medecine
10 MCS - Mayo Clinic Scottsdale
11 HSCRI - Hospital for Sick Children Research Institute
Mónica Roxo-Rosa
- Fonction : Auteur
Aleksander Edelman
- Fonction : Auteur
- PersonId : 837324
- ORCID : 0000-0001-6627-4264
- IdRef : 06150551X
Résumé
For most expression studies focusing on the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) protein, sensitive and specific antibodies (Abs) are critically needed. Several Abs have been produced commercially or by research laboratories for CFTR detection in both cell lines with heterologous or endogenous expression and native cells/tissues. Here, we review the applicability of most Abs currently in use in CF research for the biochemical and/or immunocytochemical detection of CFTR.