Skip to Main content Skip to Navigation
Journal articles

Increasing contribution of integrated forms to total HIV DNA in blood during HIV disease progression from primary infection

Abstract : BACKGROUND:In the current context of research on HIV reservoirs, offering new insights into the persistence of HIV DNA in infected cells, which prevents viral eradication, may aid in identifying cure strategies. This study aimed to describe the establishment of stable integrated forms among total HIV DNA during primary infection (PHI) and their dynamics during the natural history of infection.METHODS:Total and integrated HIV DNA were quantified in blood from 74 PHI patients and 97 recent seroconverters (<12 months following infection, "progression cohort"). The evolution of both markers over six years was modelled (mixed-effect linear models). Their predictive values for disease progression were studied (Cox models).FINDINGS:For most patients during PHI, stable integrated forms were a minority among total HIV DNA (median: 12%) and became predominant thereafter (median at AIDS stage: 100%). Both total and integrated HIV DNA increased over a six-year period. Patients from the progression cohort who reached clinical AIDS during follow-up (n = 34) exhibited higher total and integrated HIV DNA levels at seroconversion and a higher percentage of integrated forms than did slower progressors (n = 63) (median: 100% vs 44%). The integrated HIV DNA load was strongly associated with the risk of developing AIDS (aRR = 2.63, p = 0.002).INTERPRETATION:The profile of "rapid" or "slower" progression in the natural history of HIV infection appears to be determined early in the course of HIV infection. The strong predominance of unstable unintegrated forms in PHI may explain the great benefit of this early treatment, which induces a sharp decrease in total HIV DNA. FUND: French National Agency for Research on AIDS and Viral Hepatitis.
Document type :
Journal articles
Complete list of metadata

https://hal.archives-ouvertes.fr/hal-02348707
Contributor : Accord Elsevier Ccsd Connect in order to contact the contributor
Submitted on : Friday, October 22, 2021 - 4:18:49 PM
Last modification on : Saturday, October 23, 2021 - 4:06:43 AM

File

S235239641930088X.pdf
Files produced by the author(s)

Licence


Distributed under a Creative Commons Attribution - NonCommercial 4.0 International License

Identifiers

Citation

Pauline Trémeaux, Tiphaine Lenfant, Faroudy Boufassa, Asma Essat, Adeline Mélard, et al.. Increasing contribution of integrated forms to total HIV DNA in blood during HIV disease progression from primary infection. EBioMedicine, Elsevier, 2019, 41, pp.455-464. ⟨10.1016/j.ebiom.2019.02.016⟩. ⟨hal-02348707⟩

Share

Metrics

Record views

506

Files downloads

22