Skip to Main content Skip to Navigation
Journal articles

Monocarboxylate transporters, blood lactate removal after supramaximal exercise, and fatigue indexes in humans.

Abstract : The present study investigated whether muscular monocarboxylate transporter (MCT) 1 and 4 contents are related to the blood lactate removal after supramaximal exercise, fatigue indexes measured during different supramaximal exercises, and muscle oxidative parameters in 15 humans with different training status. Lactate recovery curves were obtained after a 1-min all-out exercise. A biexponential time function was then used to determine the velocity constant of the slow phase (gamma(2)), which denoted the blood lactate removal ability. Fatigue indexes were calculated during 1-min all-out (FI(AO)) and repeated 10-s (FI(Sprint)) cycling sprints. Biopsies were taken from the vastus lateralis muscle. MCT1 and MCT4 contents were quantified by Western blots, and maximal muscle oxidative capacity (V(max)) was evaluated with pyruvate + malate and glutamate + malate as substrates. The results showed that the blood lactate removal ability (i.e., gamma(2)) after a 1-min all-out test was significantly related to MCT1 content (r = 0.70, P < 0.01) but not to MCT4 (r = 0.50, P > 0.05). However, greater MCT1 and MCT4 contents were negatively related with a reduction of blood lactate concentration at the end of 1-min all-out exercise (r = -0.56, and r = -0.61, P < 0.05, respectively). Among skeletal muscle oxidative indexes, we only found a relationship between MCT1 and glutamate + malate V(max) (r = 0.63, P < 0.05). Furthermore, MCT1 content, but not MCT4, was inversely related to FI(AO) (r = -0.54, P < 0.05) and FI(Sprint) (r = -0.58, P < 0.05). We concluded that skeletal muscle MCT1 expression was associated with the velocity constant of net blood lactate removal after a 1-min all-out test and with the fatigue indexes. It is proposed that MCT1 expression may be important for blood lactate removal after supramaximal exercise based on the existence of lactate shuttles and, in turn, in favor of a better tolerance to muscle fatigue.
Document type :
Journal articles
Complete list of metadatas

Cited literature [34 references]  Display  Hide  Download

https://www.hal.inserm.fr/hal-01587472
Contributor : Dominique Mornet <>
Submitted on : Wednesday, September 29, 2010 - 3:10:16 PM
Last modification on : Friday, May 8, 2020 - 1:38:40 AM
Long-term archiving on: : Thursday, December 30, 2010 - 2:21:13 AM

Identifiers

Collections

Citation

Claire Thomas, Sabine Perrey, Karen Lambert, Gèrald Hugon, Dominique Mornet, et al.. Monocarboxylate transporters, blood lactate removal after supramaximal exercise, and fatigue indexes in humans.. Journal of Applied Physiology, American Physiological Society, 2005, 98 (3), pp.804-9. ⟨10.1152/japplphysiol.01057.2004⟩. ⟨hal-01587472v2⟩

Share

Metrics

Record views

361

Files downloads

1326