Figure 2. Dose-dependent inhibition of PrPSc formation in N2a58/22L cells by purified monoclonal antibodies SAF34 and SAF61
(A) Cells were treated with purified antibodies SAF34 and SAF61 at various concentrations for 6 days. At confluence, cells were lyzed, samples digested by proteinase K and analyzed by immunoblotting as previously described in Figure 1. Molecular weight markers spanning 16–32 kDa. (B) Densitometric measurement of PrPSc bands detected in the immunoblots. Densitometry was conducted with at least four different films from independent experiments. Values are given as densitometric units (DU) where 100% corresponds to the intensity of the PrPSc band in the absence of antibody and 0% represents undetectable levels of PrPSc in the immunoblot. (□) SAF61; (△)SAF34.