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Development of an in-vitro intestinal model featuring peristaltic motion

Abstract : My PhD work is part of the organ-on-chip field, and more precisely part of the gut-on-chip field. It is in line with the main objective of this field, which is the development of in-vitro models recapitulating as faithfully as possible the intestinal micro-environment. Through my PhD work I first developed a versatile gut-on-chip platform recapitulating the intestinal 3D architecture as well as its dynamic micro-environment. Therefore, this platform allows us to study the influence of the intestinal dynamic, especially the peristalsis, on cellular behavior in function of the 3D architecture of the scaffold. For this study Caco2 cells have been seeded either on a 2D or a 3D scaffold coated with laminin and submitted to a cyclic stretching (at 0.2 Hz and 10%) for 2, 5, 8, 16, 24 and 48 hours. Our main observation was the cellular reorientation induced by the stretching, therefore we characterized the cell behavior in function of the coating condition, the initial confluency, the stretching time and the scaffold geometry. Interestingly, the strongest cellular response was obtained when the 3D geometry and the stretching was combined illustrating the need of these two stimuli to better mimic the intestinal in vivo conditions.
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https://tel.archives-ouvertes.fr/tel-03155665
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Submitted on : Tuesday, March 2, 2021 - 1:04:57 AM
Last modification on : Thursday, March 4, 2021 - 4:10:13 PM

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  • HAL Id : tel-03155665, version 1

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Lauriane Gérémie. Development of an in-vitro intestinal model featuring peristaltic motion. Biological Physics [physics.bio-ph]. Sorbonne Université, 2019. English. ⟨NNT : 2019SORUS118⟩. ⟨tel-03155665⟩

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