Skip to Main content Skip to Navigation
Theses

Utilisation de l’ADN circulant pour l’étude de l'hétérogénéité tumorale et l'évolution clonale en oncologie pédiatrique

Abstract : Pediatric cancers represent a therapeutic challenge, and an understanding of the mechanisms of escape from treatment is necessary in order to be able to develop new therapeutic approaches. Circulating DNA can be released by a tumor in bodily fluids and enable to detect and follow genetic alterations in tumors by successive minimally invasive samples such as a blood test. In this work, a “whole exome” sequencing technique for circulating DNA was developed to allow the study of genetic tumor alterations in plasma in children with cancer. These analyzes highlight the frequent spatial and temporal genetic heterogeneity of pediatric cancers. In addition, an important role of clonal evolution in the progression of the disease was thus highlighted. Approaches using the particular characteristics of circulating DNA have also made it possible to infer the expression profile, based on the imprint of the transcription start sites, or the epigenetic profile of the tumor. In addition to an aid in the classification of tumors, these features may help to observe a change in cellular identity under treatment. Circulating DNA is therefore an important tool for better understanding mechanisms of escape from treatment of a tumor based on spatial and temporal heterogeneity and cellular plasticity.
Complete list of metadata

https://tel.archives-ouvertes.fr/tel-03154451
Contributor : Abes Star :  Contact
Submitted on : Monday, March 1, 2021 - 1:01:11 AM
Last modification on : Saturday, April 3, 2021 - 3:22:15 AM

File

97423_CHICARD_2020_archivage.p...
Version validated by the jury (STAR)

Identifiers

  • HAL Id : tel-03154451, version 1

Collections

Citation

Mathieu Chicard. Utilisation de l’ADN circulant pour l’étude de l'hétérogénéité tumorale et l'évolution clonale en oncologie pédiatrique. Cancer. Université Paris-Saclay, 2020. Français. ⟨NNT : 2020UPASL002⟩. ⟨tel-03154451⟩

Share

Metrics

Record views

145

Files downloads

77