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Epithelial folding and migration

Abstract : In many developmental contexts, epithelial folding is accompanied by neighboring tissue flows towards the invagination site. To assess the idea of a long-range coupling between invagination and tissue flow, we have studied two macroscopic morphogenetic events happening during Drosophila metamorphosis: the invagination of the head-to-thorax boundary and the anterior dorsal thorax flow. Using large-scale laser ablations or locally disrupting MyosinII contractility in the invaginating region, we have found that head-thorax interface invagination is an active process which is providing a pulling force locally driving thorax flow. However, we have discovered that thorax flow is also partly autonomous, and thereby can actively provide a pushing force towards the invagination site. Using forward genetic methods, we have uncovered that thorax flow rely on collective migration on the apical extracellular matrix (ECM), depending on the secretion of the ZP protein Dumpy and on planar polarity cues. We have furthermore shown in vivo that regions of active thorax migration are specifically associated with low apical ECM deformation. Taken together, my PhD work uncovers a new mechanism coordinating tissue folding and tissue flow, and emphasizes the importance of tissue-matrix interaction in epithelial mechanics and morphogenesis.
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Submitted on : Friday, February 12, 2021 - 12:37:49 PM
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VILLEDIEU_Aurelien_2019.pdf
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  • HAL Id : tel-03139853, version 1

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Aurélien Villedieu. Epithelial folding and migration. Cellular Biology. Sorbonne Université, 2019. English. ⟨NNT : 2019SORUS400⟩. ⟨tel-03139853⟩

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