Temperature-Switchable Control of Ligand Display on Adlayers of Mixed Poly(lysine)- g -(PEO) and Poly(lysine)- g -(ligand-modified poly- N -isopropylacrylamide)

Abstract : Adlayers of poly(lysine)-g-PEG comblike copolymer are extensively used to prepare cell-repellant and protein-repellent surfaces by a straightforward coulomb-driven adsorption that is compatible with diverse substrates (glass, Petri dish, etc.). To endow surfaces with functional properties, namely, controlled ligand-protein binding, comblike poly(lysine) derivatives were used to deposit temperature-responsive poly(NIPAM) macrografts mixed with PEG ones on glass surfaces. Simple surface immersion in mixed solutions of biotin-modified poly(lysine)-g-poly(N-isopropylacrylamide) and poly(lysine)-g-poly(ethylene oxide) yielded robust adlayers whose composition reflected the ratio between the two polymers in solution. We show by fluorescence imaging, and comparison with repellent 100% PEGylated patterns, that specific binding of model avidin/particle conjugates (diameters of ca. 10 or 200 nm) was controlled by temperature switch. The biotin ligand was displayed and accessible at low T, or hidden at T > LCST. Topography and mechanical mapping measurements by AFM confirmed the swelling/collapse status of PNIPAM macrografts in the adlayer at low/high T, respectively. Temperature-responsive comblike PLL derivative that can spontaneously cover anionic interfaces is a promising platform enabling good control on the deposition and accessibility of biofunctional groups on various solid surfaces.
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Article dans une revue
Biomacromolecules, American Chemical Society, 2016, 17 (5), pp.1727-1736. <10.1021/acs.biomac.6b00136>
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Soumis le : mercredi 19 avril 2017 - 13:45:19
Dernière modification le : lundi 29 mai 2017 - 14:32:30

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F. Dalier, F. Eghiaian, S. Scheuring, E. Marie, C. Tribet. Temperature-Switchable Control of Ligand Display on Adlayers of Mixed Poly(lysine)- g -(PEO) and Poly(lysine)- g -(ligand-modified poly- N -isopropylacrylamide). Biomacromolecules, American Chemical Society, 2016, 17 (5), pp.1727-1736. <10.1021/acs.biomac.6b00136>. <hal-01510306>

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