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Journal Articles Biochimie Year : 2022

Guidelines for G-quadruplexes: I. In vitro characterization


Besides the well-known DNA double-helix, non-canonical nucleic acid structures regulate crucial bio- logical activities. Among these oddities, guanine-rich DNA sequences can form unusual four-stranded secondary structures called G-quadruplexes (G4s). G4-prone sequences have been found in the ge- nomes of most species, and G4s play important roles in essential processes such as transcription, replication, genome integrity and epigenetic regulation. Here, we present a short overview of G-quad- ruplexes followed by a detailed description of the biophysical and biochemical methods used to char- acterize G4s in vitro. The principles, experimental details and possible shortcomings of each method are discussed to provide a comprehensive view of the techniques used to study these structures. We aim to provide a set of guidelines for standardizing research on G-quadruplexes; these guidelines are not meant to be a dogmatic set of rules, but should rather provide useful information on the methods currently used to study these fascinating motifs.


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inserm-03979024 , version 1 (08-02-2023)



Yu Luo, Anton Granzhan, Julien Marquevielle, Anne Cucchiarini, Laurent Lacroix, et al.. Guidelines for G-quadruplexes: I. In vitro characterization. Biochimie, 2022, pp.S0300-9084(22)00344-3. ⟨10.1016/j.biochi.2022.12.019⟩. ⟨inserm-03979024⟩
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