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Journal Articles Nature Communications Year : 2022

Deciphering the heterogeneity of the Lyve1+ perivascular macrophages in the mouse brain

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R Stumm

Abstract

Perivascular macrophages (pvMs) are associated with cerebral vasculature and mediate brain drainage and immune regulation. Here, using reporter mouse models, whole brain and section immunofluorescence, flow cytometry, and single cell RNA sequencing, besides the Lyve1 + F4/80 + CD206 + CX3CR1 + pvMs, we identify a CX3CR1-pvM population that shares phagocytic functions and location. Furthermore, the brain parenchyma vasculature mostly hosts Lyve1 + MHCII-pvMs with low to intermediate CD45 expression. Using the double Cx3cr1 GFP x Cx3cr1-Cre;Rosa tdT reporter mice for finer mapping of the lineages, we establish that CD45 low CX3CR1-pvMs are derived from CX3CR1 + precursors and require PU.1 during their ontogeny. In parallel, results from the Cxcr4-CreErt2;Rosa26 tdT lineage tracing model support a bone marrowindependent replenishment of all Lyve1 + pvMs in the adult mouse brain. Lastly, flow cytometry and 3D immunofluorescence analysis uncover increased percentage of pvMs following photothrombotic induced stroke. Our results thus show that the parenchymal pvM population is more heterogenous than previously described, and includes a CD45 low and CX3CR1-pvM population.
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Origin : Publication funded by an institution
Licence : CC BY NC - Attribution - NonCommercial

Dates and versions

inserm-03945391 , version 1 (18-01-2023)

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C Siret, M van Lessen, J Bavais, H W Jeong, S K Reddy Samawar, et al.. Deciphering the heterogeneity of the Lyve1+ perivascular macrophages in the mouse brain. Nature Communications, 2022, 13 (1), pp.7366. ⟨10.1038/s41467-022-35166-9⟩. ⟨inserm-03945391⟩
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