Tetraspanin CD53 controls T cell immunity through regulation of CD45RO stability, mobility, and function - Archive ouverte HAL Access content directly
Journal Articles Cell Reports Year : 2022

Tetraspanin CD53 controls T cell immunity through regulation of CD45RO stability, mobility, and function

(1) , (1) , (1) , (2) , (3) , (1) , (1) , (1) , (1) , (1) , (4, 5) , (4, 5) , (6) , (6) , (1) , (2) , (1)
1
2
3
4
5
6

Abstract

T cells depend on the phosphatase CD45 to initiate T cell receptor signaling. Although the critical role of CD45 in T cells is established, the mechanisms controlling function and localization in the membrane are not well understood. Moreover, the regulation of specific CD45 isoforms in T cell signaling remains unresolved. By using unbiased mass spectrometry, we identify the tetraspanin CD53 as a partner of CD45 and show that CD53 controls CD45 function and T cell activation. CD53-negative T cells (Cd53-/-) exhibit substantial proliferation defects, and Cd53-/- mice show impaired tumor rejection and reduced IFNγ-producing T cells compared with wild-type mice. Investigation into the mechanism reveals that CD53 is required for CD45RO expression and mobility. In addition, CD53 is shown to stabilize CD45 on the membrane and is required for optimal phosphatase activity and subsequent Lck activation. Together, our findings reveal CD53 as a regulator of CD45 activity required for T cell immunity.
Fichier principal
Vignette du fichier
Dunlock Cell Reports 2022.pdf (4.24 Mo) Télécharger le fichier
Origin : Publication funded by an institution

Dates and versions

inserm-03826292 , version 1 (24-10-2022)

Identifiers

Cite

Vera-Marie E Dunlock, Abbey B Arp, Simar Pal Singh, Stéphanie Charrin, Viet Nguyen, et al.. Tetraspanin CD53 controls T cell immunity through regulation of CD45RO stability, mobility, and function. Cell Reports, 2022, 39 (13), pp.111006. ⟨10.1016/j.celrep.2022.111006⟩. ⟨inserm-03826292⟩
0 View
0 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More