Translation in astrocyte distal processes sets molecular heterogeneity at the gliovascular interface - Archive ouverte HAL Access content directly
Journal Articles Cell Discovery Year : 2017

Translation in astrocyte distal processes sets molecular heterogeneity at the gliovascular interface

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Corinne Blugeon
  • Function : Author
Sandrine Perrin
  • Function : Author
Juliette Pouch
  • Function : Author

Abstract

Astrocytes send out long processes that are terminated by endfeet at the vascular surface and regulate vascular functions as well as homeostasis at the vascular interface. To date, the astroglial mechanisms underlying these functions have been poorly addressed. Here we demonstrate that a subset of messenger RNAs is distributed in astrocyte endfeet. We identified, among this transcriptome, a pool of messenger RNAs bound to ribosomes, the endfeetome, that primarily encodes for secreted and membrane proteins. We detected nascent protein synthesis in astrocyte endfeet. Finally, we determined the presence of smooth and rough endoplasmic reticulum and the Golgi apparatus in astrocyte perivascular processes and endfeet, suggesting for local maturation of membrane and secreted proteins. These results demonstrate for the first time that protein synthesis occurs in astrocyte perivascular distal processes that may sustain their structural and functional polarization at the vascular interface.
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Dates and versions

inserm-03823110 , version 1 (20-10-2022)

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Anne- Cécile Boulay, Bruno Saubaméa, Nicolas Adam, Stéphanie Chasseigneaux, Noémie Mazaré, et al.. Translation in astrocyte distal processes sets molecular heterogeneity at the gliovascular interface. Cell Discovery, 2017, 3 (1), pp.17005. ⟨10.1038/celldisc.2017.5⟩. ⟨inserm-03823110⟩
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