G-quadruplex structure of the C. elegans telomeric repeat: a two tetrads basket type conformation stabilized by a non-canonical C–T base-pair - Inserm - Institut national de la santé et de la recherche médicale Accéder directement au contenu
Article Dans Une Revue Nucleic Acids Research Année : 2022

G-quadruplex structure of the C. elegans telomeric repeat: a two tetrads basket type conformation stabilized by a non-canonical C–T base-pair

Résumé

The Caenorhabditis elegans model has greatly contributed to the understanding of the role of G-quadruplexes in genomic instability. The GGCTTA repeats of the C. elegans telomeres resemble the GGGTTA repeats of the human telomeres. However, the comparison of telomeric sequences (Homo sapiens, Tetrahymena, Oxytricha, Bombyx mori and Giardia) revealed that small changes in these repeats can drastically change the topology of the folded G-quadruplex. In the present work we determined the structure adopted by the C. elegans telomeric sequence d[GG(CTTAGG)3]. The investigated C. elegans telomeric sequence is shown to fold into an intramolecular two G-tetrads basket type G-quadruplex structure that includes a C–T base pair in the diagonal loop. This work sheds light on the telomeric structure of the widely used C. elegans animal model.
Fichier principal
Vignette du fichier
gkac523.pdf (4.88 Mo) Télécharger le fichier
Origine : Publication financée par une institution

Dates et versions

inserm-03807354 , version 1 (09-10-2022)

Identifiants

Citer

Julien Marquevielle, Aurore de Rache, Brune Vialet, Estelle Morvan, Jean-Louis Mergny, et al.. G-quadruplex structure of the C. elegans telomeric repeat: a two tetrads basket type conformation stabilized by a non-canonical C–T base-pair. Nucleic Acids Research, 2022, 50 (12), pp.7134-7146. ⟨10.1093/nar/gkac523⟩. ⟨inserm-03807354⟩
10 Consultations
34 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More