The DCMU Herbicide Shapes T-cell Functions By Modulating Micro-RNA Expression Profiles - Archive ouverte HAL Access content directly
Journal Articles Frontiers in Immunology Year : 2022

The DCMU Herbicide Shapes T-cell Functions By Modulating Micro-RNA Expression Profiles

(1, 2) , (1, 2) , (3) , (1, 2) , (4) , (4) , (3) , (1, 2) , (1, 2) , (1, 2)
1
2
3
4

Abstract

DCMU [N-(3,4-dichlorophenyl)-N-dimethylurea] or diuron is a widely used herbicide, which can cause adverse effects on human, especially on immune cells, due to their intrinsic properties and wide distribution. These cells are important for fighting not only against virus or bacteria but also against neoplastic cell development. We developed an approach that combines functional studies and miRNA and RNA sequencing data to evaluate the effects of DCMU on the human immune response against cancer, particularly the one carried out by CD8+ T cells. We found that DCMU modulates the expression of miRNA in a dose-dependent manner, leading to a specific pattern of gene expression and consequently to a diminished cytokine and granzyme B secretions. Using mimics or anti-miRs, we identified several miRNA, such as hsa-miR-3135b and hsa-miR-21-5p, that regulate these secretions. All these changes reduce the CD8+ T cells’ cytotoxic activity directed against cancer cells, in vitro and in vivo in a zebrafish model. To conclude, our study suggests that DCMU reduces T-cell abilities, participating thus to the establishment of an environment conducive to cancer development.
Fichier principal
Vignette du fichier
fimmu-13-925241.pdf (4.37 Mo) Télécharger le fichier
Origin : Publisher files allowed on an open archive

Dates and versions

inserm-03752032 , version 1 (16-08-2022)

Identifiers

Cite

Pierre Autin, Sophie Deshayes, Juliette Lea, Nicolas Boisgerault, Emilie Dupré, et al.. The DCMU Herbicide Shapes T-cell Functions By Modulating Micro-RNA Expression Profiles. Frontiers in Immunology, 2022, 13, pp.925241. ⟨10.3389/fimmu.2022.925241⟩. ⟨inserm-03752032⟩
42 View
3 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More