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The beginning and the end of SNARE‐induced membrane fusion

Abstract : Membrane fusion is not a spontaneous process. Physiologically, the formation of coiled-coil protein complexes, the SNAREpins, bridges the membrane of a vesicle and a target membrane, brings them in close contact and provides the energy necessary for their fusion. In this review, we utilize results from in vitro experiments and simple physics and chemistry models to dissect the kinetics and energetics of the fusion process from the encounter of the two membranes to the full expansion of a fusion pore. We find three main energy barriers that oppose the fusion process: SNAREpin initiation, fusion pore opening and expansion. SNAREpin initiation is inherent to the proteins and makes in vitro fusion kinetics experiments rather slow. The kinetics are physiologically accelerated by effectors. The energy barriers that precede pore opening and pore expansion can be overcome by several SNAREpin acting in concert.
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Submitted on : Wednesday, June 1, 2022 - 11:50:51 AM
Last modification on : Sunday, June 5, 2022 - 3:10:45 AM

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Delphine Mion, Louis Bunel, Paul Heo, Frédéric Pincet. The beginning and the end of SNARE‐induced membrane fusion. FEBS Open Bio, Wiley Open Access/Elsevier, 2022, Online ahead of print. ⟨10.1002/2211-5463.13447⟩. ⟨inserm-03684267⟩

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