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Induction of Human Naïve Pluripotent Stem Cells from Somatic Cells

Abstract : Generating patient-specific stem cells representing the onset of development has become possible since the discovery of somatic cell reprogramming into induced pluripotent stem cells. However, human pluripotent stem cells are generally cultured in a primed pluripotent state: they are poised for differentiation and represent a stage of development corresponding to post-implantation epiblast. Here, we describe a protocol to reprogram human fibroblasts into naive pluripotent stem cells by overexpressing the transcription factors OCT4, SOX2, KLF4, and c-MYC using Sendai viruses. The resulting cells represent an earlier stage of development that corresponds to pre-implantation epiblast. We also discuss validation methods for human naive pluripotent stem cells.
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Submitted on : Friday, April 29, 2022 - 2:55:49 PM
Last modification on : Monday, July 18, 2022 - 8:24:03 AM
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Constance Onfray, Jia Ping Tan, Stéphanie Kilens, Xiaodong Liu, Jose Polo, et al.. Induction of Human Naïve Pluripotent Stem Cells from Somatic Cells. Human Naïve Pluripotent Stem Cells, 2416, Springer US, pp.39-51, 2022, Methods in Molecular Biology, ⟨10.1007/978-1-0716-1908-7_4⟩. ⟨inserm-03655377⟩



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