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Book Sections Year : 2022

Induction of Human Naïve Pluripotent Stem Cells from Somatic Cells

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Abstract

Generating patient-specific stem cells representing the onset of development has become possible since the discovery of somatic cell reprogramming into induced pluripotent stem cells. However, human pluripotent stem cells are generally cultured in a primed pluripotent state: they are poised for differentiation and represent a stage of development corresponding to post-implantation epiblast. Here, we describe a protocol to reprogram human fibroblasts into naive pluripotent stem cells by overexpressing the transcription factors OCT4, SOX2, KLF4, and c-MYC using Sendai viruses. The resulting cells represent an earlier stage of development that corresponds to pre-implantation epiblast. We also discuss validation methods for human naive pluripotent stem cells.
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Dates and versions

inserm-03655377 , version 1 (29-04-2022)

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Constance Onfray, Jia Ping Tan, Stéphanie Kilens, Xiaodong Liu, Jose Polo, et al.. Induction of Human Naïve Pluripotent Stem Cells from Somatic Cells. Human Naïve Pluripotent Stem Cells, 2416, Springer US, pp.39-51, 2022, Methods in Molecular Biology, ⟨10.1007/978-1-0716-1908-7_4⟩. ⟨inserm-03655377⟩
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