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Methods for the identification and characterization of extracellular vesicles in cardiovascular studiesfrom exosomes to microvesicles

Sean Davidson 1 Chantal Boulanger 2 Elena Aikawa 3 Lina Badimon 4 Lucio Barile 5 Christoph Binder 6 Alain Brisson 7 Edit Buzas 8 Costanza Emanueli 9 Felix Jansen 10 Miroslava Katsur 1 Romaric Lacroix 11, 12 Sai Lim 13, 14 Nigel Mackman 15 Manuel Mayr 16 Philippe Menasché 17, 18 Rienk Nieuwland 19 Susmita Sahoo 20 Kaloyan Takov 16 Thomas Thum 21, 22 Pieter Vader 2, 23 Marca Wauben 18 Kenneth Witwer 24 Joost Sluijter 18 
Abstract : Extracellular vesicles (EVs) are nanosized vesicles with a lipid bilayer that are released from cells of the cardiovascular system, and are considered important mediators of intercellular and extracellular communication. Two types of EV of particular interest are exosomes and microvesicles, which have been identified in all tissue and body fluids and carry a variety of molecules including RNAs, proteins, and lipids. EVs have potential for use in the diagnosis and prognosis of cardiovascular diseases and as new therapeutic agents, particularly in the setting of myocardial infarction and heart failure. Despite their promise, technical challenges related to their small size make it challenging to accurately identify and characterize them, and to study EV-mediated processes. Here, we aim to provide the reader with an overview of the techniques and technologies available for the separation and characterization of EVs from different sources. Methods for determining the protein, RNA and lipid content of EVs are discussed. The aim of this document is to provide guidance on critical methodological issues and highlight key points for consideration for the investigation of EVs in cardiovascular studies.
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Submitted on : Friday, March 25, 2022 - 5:05:35 PM
Last modification on : Thursday, April 7, 2022 - 1:58:22 PM
Long-term archiving on: : Sunday, June 26, 2022 - 7:10:39 PM


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Sean Davidson, Chantal Boulanger, Elena Aikawa, Lina Badimon, Lucio Barile, et al.. Methods for the identification and characterization of extracellular vesicles in cardiovascular studiesfrom exosomes to microvesicles. Cardiovascular Research, Oxford University Press (OUP), In press, Online ahead of print. ⟨10.1093/cvr/cvac031⟩. ⟨inserm-03620308⟩



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