Flow cytometric analysis of myelodysplasia: Pre‐analytical and technical issues—Recommendations from the European LeukemiaNet
Vincent Velden
(1)
,
Frank Preijers
(2)
,
Ulrika Johansson
(3)
,
Theresia Westers
(4)
,
Alan Dunlop
(5)
,
Anna Porwit
(6)
,
Marie Béné
(7, 8)
,
Peter Valent
(9)
,
Jeroen Marvelde
(1)
,
Orianne Wagner‐ballon
(10, 11)
,
Uta Oelschlaegel
(12)
,
Leonie Saft
(13)
,
Sharham Kordasti
(14)
,
Robin Ireland
(14)
,
Eline Cremers
(15)
,
Canan Alhan
(4)
,
Carolien Duetz
(4)
,
Willemijn Hobo
(2)
,
Nicolas Chapuis
(16, 17)
,
Michaela Fontenay
(16, 17)
,
Peter Bettelheim
(18)
,
Lisa Eidenshink‐brodersen
(19)
,
Patricia Font
(20)
,
Michael Loken
(19)
,
Sergio Matarraz
(21, 22)
,
Kiyoyuki Ogata
(23)
,
Alberto Orfao
(22, 21)
,
Katherina Psarra
(24)
,
Dolores Subirá
(25)
,
Denise Wells
(19)
,
Matteo Della Porta
(26)
,
Kate Burbury
(27)
,
Frauke Bellos
(28)
,
Elisabeth Weiss
(28)
,
Wolfgang Kern
(28)
,
Arjan Loosdrecht
(4)
1
Erasmus MC -
Erasmus University Medical Center [Rotterdam]
2 Radboudumc Nijmegen [The Netherlands]
3 University Hospitals Bristol
4 Amsterdam UMC - Amsterdam University Medical Center
5 Royal Marsden Hospital [Surrey, UK]
6 Lund University [Lund]
7 CRCINA-ÉQUIPE 1 - Immunobiology of Human αβ and γδ T Cells and Immunotherapeutic Applications
8 CHU Nantes - Centre hospitalier universitaire de Nantes
9 Medizinische Universität Wien = Medical University of Vienna
10 IMRB - Institut Mondor de Recherche Biomédicale
11 CHU Henri Mondor [Créteil]
12 University Hospital Carl Gustav Carus [Dresden, Germany]
13 Karolinska Institutet [Stockholm]
14 King‘s College London
15 Maastricht University [Maastricht]
16 Hôpital Cochin [AP-HP]
17 IC UM3 (UMR 8104 / U1016) - Institut Cochin
18 Ordensklinikum Linz Elisabethinen
19 HematoLogics, Inc. [Seattle, WA, USA]
20 Hospital General Universitario "Gregorio Marañón" [Madrid]
21 Universidad de Salamanca
22 ISC - Instituto de Salud Carlos III [Madrid]
23 MRTC Japan - Metropolitan Research and Treatment Centre for Blood Disorders [Tokyo, Japan]
24 Evangelismos Athens General Hospital
25 Universidad de Guadalajara
26 Hunimed - Humanitas University [Milan]
27 University of Melbourne
28 Munich Leukemia Laboratory [Munich, Germany]
2 Radboudumc Nijmegen [The Netherlands]
3 University Hospitals Bristol
4 Amsterdam UMC - Amsterdam University Medical Center
5 Royal Marsden Hospital [Surrey, UK]
6 Lund University [Lund]
7 CRCINA-ÉQUIPE 1 - Immunobiology of Human αβ and γδ T Cells and Immunotherapeutic Applications
8 CHU Nantes - Centre hospitalier universitaire de Nantes
9 Medizinische Universität Wien = Medical University of Vienna
10 IMRB - Institut Mondor de Recherche Biomédicale
11 CHU Henri Mondor [Créteil]
12 University Hospital Carl Gustav Carus [Dresden, Germany]
13 Karolinska Institutet [Stockholm]
14 King‘s College London
15 Maastricht University [Maastricht]
16 Hôpital Cochin [AP-HP]
17 IC UM3 (UMR 8104 / U1016) - Institut Cochin
18 Ordensklinikum Linz Elisabethinen
19 HematoLogics, Inc. [Seattle, WA, USA]
20 Hospital General Universitario "Gregorio Marañón" [Madrid]
21 Universidad de Salamanca
22 ISC - Instituto de Salud Carlos III [Madrid]
23 MRTC Japan - Metropolitan Research and Treatment Centre for Blood Disorders [Tokyo, Japan]
24 Evangelismos Athens General Hospital
25 Universidad de Guadalajara
26 Hunimed - Humanitas University [Milan]
27 University of Melbourne
28 Munich Leukemia Laboratory [Munich, Germany]
Vincent Velden
- Function : Author
- PersonId : 821107
- ORCID : 0000-0001-9457-3763
Ulrika Johansson
- Function : Author
- PersonId : 767741
- ORCID : 0000-0003-0743-7400
Marie Béné
- Function : Author
- PersonId : 767851
- ORCID : 0000-0002-6569-7414
Peter Valent
- Function : Author
- PersonId : 759814
- ORCID : 0000-0003-0456-5095
Orianne Wagner‐ballon
- Function : Author
- PersonId : 756203
- ORCID : 0000-0001-9071-6683
- IdRef : 079569668
Nicolas Chapuis
- Function : Author
- PersonId : 768515
- ORCID : 0000-0003-0601-3948
- IdRef : 12957189X
Michaela Fontenay
- Function : Author
- PersonId : 770387
- ORCID : 0000-0002-5492-6349
- IdRef : 083817247
Alberto Orfao
- Function : Author
- PersonId : 759817
- ORCID : 0000-0002-0007-7230
Dolores Subirá
- Function : Author
- PersonId : 762164
- ORCID : 0000-0002-9485-0529
Abstract
Background: Flow cytometry (FCM) aids the diagnosis and prognostic stratification of patients with suspected or confirmed myelodysplastic syndrome (MDS). Over the past few years, significant progress has been made in the FCM field concerning technical issues (including software and hardware) and pre-analytical procedures.
Methods: Recommendations are made based on the data and expert discussions generated from 13 yearly meetings of the European LeukemiaNet international MDS Flow working group.
Results: We report here on the experiences and recommendations concerning (1) the optimal methods of sample processing and handling, (2) antibody panels and fluorochromes, and (3) current hardware technologies.
Conclusions: These recommendations will support and facilitate the appropriate application of FCM assays in the diagnostic workup of MDS patients. Further standardization and harmonization will be required to integrate FCM in MDS diagnostic evaluations in daily practice.
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Origin : Publication funded by an institution