Ultrasound Neurostimulation in Mice: Impact of Ultrasound Settings and Beam Properties - Inserm - Institut national de la santé et de la recherche médicale Access content directly
Journal Articles IEEE Transactions on Ultrasonics, Ferroelectrics and Frequency Control Year : 2022

Ultrasound Neurostimulation in Mice: Impact of Ultrasound Settings and Beam Properties

Jean-Michel Escoffre
Ayache Bouakaz
Tarik Iazourène
  • Function : Author
  • PersonId : 1123352
Hanaa Malloul
  • Function : Author
  • PersonId : 1123353
Rasha Noureddine
  • Function : Author
  • PersonId : 1123354

Abstract

Ultrasound neurostimulation (USNS) is being investigated as a treatment approach for neuropsychiatric and neurodegenerative disorders. Indeed, unlike the existing methods that use electric or magnetic stimulation, it offers the possibility to modulate brain activity in a non-invasive way, with a good spatial specificity and a high penetration capacity. However, there is no consensus yet on ultrasound parameters and beam properties required for an efficient neurostimulation. In this context, this preclinical study aimed to elucidate the effect of frequency, peak negative pressure (PNP), pulse duration (PD) and focal spot diameter, on the USNS efficiency. This was done by targeting the motor cortex (M1) of seventy healthy mice and analyzing the elicited motor responses (visually and with electromyography). Also, a further investigation was performed by assessing the corresponding neuronal activity, using c-Fos immunostaining. The results showed that the success rate, a metric that depicts USNS efficacy, increased with PNP in a sigmoidal way, reaching up to 100 %. This was verified at different frequencies (0.5, 1, 1.5 and 2.25 MHz) and pulse durations (53.3, 160 and 320 ms, at 1.5 MHz fixed frequency). Moreover, it was shown that higher PNP values were required to achieve a constant USNS efficacy not only when frequency increased, but also when the focal spot diameter decreased, emphasizing a close link between these acoustic parameters and USNS efficacy. These findings were confirmed with immunohistochemistry, which showed a strong relationship between neural activation, the applied PNP and the focal spot diameter.
Embargoed file
Embargoed file
Ne sera jamais visible

Dates and versions

inserm-03528841 , version 1 (17-01-2022)

Identifiers

  • HAL Id : inserm-03528841 , version 1

Cite

Jean-Michel Escoffre, Ayache Bouakaz, Tarik Iazourène, Hanaa Malloul, Edward Oujagir, et al.. Ultrasound Neurostimulation in Mice: Impact of Ultrasound Settings and Beam Properties. IEEE Transactions on Ultrasonics, Ferroelectrics and Frequency Control, 2022, Ahead of print. ⟨inserm-03528841⟩

Collections

INSERM UNIV-TOURS
36 View
25 Download

Share

Gmail Facebook Twitter LinkedIn More