Skip to Main content Skip to Navigation
Journal articles

Relating polymeric microparticle formulation to prevalence or distribution of fibronectin and poly- d -lysine to support mesenchymal stem cell growth

Abstract : Protein-coated polymer-based microparticles are attractive supports for cell delivery, but the interplay between microparticle properties, protein coating, and cell response is poorly understood. The interest in alternative microparticle formulations increases the need for a better understanding of how functional protein coatings form on different microparticles. In this work, microparticle formulations based on biodegradable polymers [poly (lactic-co-glycolic acid) (PLGA) and the triblock copolymer PLGA-poloxamer-PLGA] were prepared via an emulsion-based process. To explore the impact that the use of a surfactant has on the properties of the microparticles, the emulsion was stabilized by using either a surfactant, poly(vinyl alcohol), or an organic solvent, propylene glycol. Four different types of microparticles were prepared through combinations of the two types of polymers and the two types of stabilizers. The coating of microparticles with proteins/polypeptides such as fibronectin and poly-d-lysine has been demonstrated before and is an integral step for their application as microcarriers, e.g., for cell delivery; however, the impact of the microparticles' surface chemical properties on the formation (prevalence and distribution) of the mixed polypeptide coatings and the influence on subsequent cell attachment remain to be elucidated. Using a colocalization analysis approach on ToF-SIMS images of protein-coated microparticles, we show that the use of propyleneglycol over PVA as well as the substitution of PLGA by the triblock copolymer resulted in enhanced protein adsorption. Furthermore, if propyleneglycol is used, the substitution of PLGA with the triblock copolymer leads to increased stem cell adhesion.
Document type :
Journal articles
Complete list of metadata

https://www.hal.inserm.fr/inserm-03498875
Contributor : Elizabeth Bernardo Connect in order to contact the contributor
Submitted on : Tuesday, December 21, 2021 - 11:25:42 AM
Last modification on : Wednesday, January 19, 2022 - 5:06:01 PM

Identifiers

Collections

Citation

Deniz Ugur, Virginie Sottile, Claudia Montero-Menei, Franck Boury, Mischa Zelzer. Relating polymeric microparticle formulation to prevalence or distribution of fibronectin and poly- d -lysine to support mesenchymal stem cell growth. Biointerphases, American Vacuum Society, 2020, 15 (4), pp.041008. ⟨10.1116/6.0000226⟩. ⟨inserm-03498875⟩

Share

Metrics

Les métriques sont temporairement indisponibles