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Proteomic Identification of an Endogenous Synaptic SUMOylome in the Developing Rat Brain

Abstract : Synapses are highly specialized structures that interconnect neurons to form functional networks dedicated to neuronal communication. During brain development, synapses undergo activity-dependent rearrangements leading to both structural and functional changes. Many molecular processes are involved in this regulation, including post-translational modifications by the Small Ubiquitin-like MOdifier SUMO. To get a wider view of the panel of endogenous synaptic SUMO-modified proteins in the mammalian brain, we combined subcellular fractionation of rat brains at the post-natal day 14 with denaturing immunoprecipitation using SUMO2/3 antibodies and tandem mass spectrometry analysis. Our screening identified 803 candidate SUMO2/3 targets, which represents about 18% of the synaptic proteome. Our dataset includes neurotransmitter receptors, transporters, adhesion molecules, scaffolding proteins as well as vesicular trafficking and cytoskeleton-associated proteins, defining SUMO2/3 as a central regulator of the synaptic organization and function.
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https://www.hal.inserm.fr/inserm-03494822
Contributor : Stephane Martin Connect in order to contact the contributor
Submitted on : Monday, December 20, 2021 - 8:19:26 AM
Last modification on : Thursday, August 4, 2022 - 4:57:43 PM

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Marie Pronot, Félicie Kieffer, Anne-Sophie Gay, Delphine Debayle, Raphaël Forquet, et al.. Proteomic Identification of an Endogenous Synaptic SUMOylome in the Developing Rat Brain. Frontiers in Molecular Neuroscience, Frontiers Media, 2021, 14, pp.780535. ⟨10.3389/fnmol.2021.780535⟩. ⟨inserm-03494822⟩

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