Service interruption on Monday 11 July from 12:30 to 13:00: all the sites of the CCSD (HAL, EpiSciences, SciencesConf, AureHAL) will be inaccessible (network hardware connection).
Skip to Main content Skip to Navigation
Journal articles

Antagonistic effects of finerenone and spironolactone on the aldosterone‐regulated transcriptome of human kidney cells

Abstract : Aldosterone, the main mineralocorticoid hormone in humans, plays a pivotal role in the control of water and salt reabsorption via activation of the mineralocorticoid receptor (MR). Alterations in MR signaling pathway lead to renal dysfunction, including chronic kidney disease and renal fibrosis, that can be prevented or treated with mineralocorticoid receptor antagonists (MRAs). Here, we used RNA-Sequencing to analyze effects of two MRAs, spironolactone and finerenone, on the aldosterone-induced transcriptome of a human renal cell line stably expressing the MR. Bioinformatics analysis of the data set reveals the identity of hundreds of genes induced or repressed by aldosterone. Their regulation is modulated in a time-dependent manner and, for the induced genes, depends on the aldosterone-driven direct binding of the MR onto its genomic targets that we have previously characterized. Although both MRAs block aldosterone-induced as well as aldosterone-repressed genes qualitatively similarly, finerenone has a quantitatively more efficient antagonism on some aldosterone-induced genes. Our data provide the first complete transcriptome for aldosterone on a human renal cell line and identifies pro-inflammatory markers (IL6, IL11, CCL7, and CXCL8) as aldosterone-repressed genes.
Complete list of metadata
Contributor : Jerome Fagart Connect in order to contact the contributor
Submitted on : Thursday, December 16, 2021 - 4:23:13 PM
Last modification on : Thursday, December 23, 2021 - 3:34:08 AM
Long-term archiving on: : Thursday, March 17, 2022 - 7:29:41 PM


 Restricted access
To satisfy the distribution rights of the publisher, the document is embargoed until : jamais

Please log in to resquest access to the document



Florian Le Billan, Julie Perrot, Elena Carceller, Simon Travers, Say Viengchareun, et al.. Antagonistic effects of finerenone and spironolactone on the aldosterone‐regulated transcriptome of human kidney cells. FASEB Journal, Federation of American Society of Experimental Biology, 2021, 35 (2), pp.e21314. ⟨10.1096/fj.202002043RR⟩. ⟨inserm-03483685⟩



Record views