Quantifying the Antiviral Effect of IFN on HIV-1 Replication in Cell Culture - Archive ouverte HAL Access content directly
Journal Articles Scientific Reports Year : 2015

Quantifying the Antiviral Effect of IFN on HIV-1 Replication in Cell Culture

(1) , (2) , (2) , (2) , (2) , (3) , (1) , (2)
1
2
3

Abstract

Type-I interferons (IFNs) induce the expression of hundreds of cellular genes, some of which have direct antiviral activities. Although IFNs restrict different steps of HIV replication cycle, their dominant antiviral effect remains unclear. We first quantified the inhibition of HIV replication by IFN in tissue culture, using viruses with different tropism and growth kinetics. By combining experimental and mathematical analyses, we determined quantitative estimates for key parameters of HIV replication and inhibition, and demonstrate that IFN mainly inhibits de novo infection (33% and 47% for a X4-and a R5-strain, respectively), rather than virus production (15% and 6% for the X4 and R5 strains, respectively). This finding is in agreement with patient-derived data analyses.
Fichier principal
Vignette du fichier
Ikeda.pdf (471.23 Ko) Télécharger le fichier
Origin : Publisher files allowed on an open archive

Dates and versions

inserm-03451963 , version 1 (26-11-2021)

Identifiers

Cite

Hiroki Ikeda, Ana Godinho-Santos, Sylvie Rato, Bénédicte Vanwalscappel, François Clavel, et al.. Quantifying the Antiviral Effect of IFN on HIV-1 Replication in Cell Culture. Scientific Reports, 2015, 5 (1), pp.11761. ⟨10.1038/srep11761⟩. ⟨inserm-03451963⟩
6 View
17 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More