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Pilot Study Assessing the Impact of Intrathecal Administration of Variants AAV-PHP.B and AAV-PHP.eB on Brain Transduction in Adult Rhesus Macaques

Abstract : Adeno-associated virus (AAV) vectors are increasingly used as an effective and safe approach to deliver genetic material to the central nervous system (CNS). The AAV9-derived variants, AAV-PHP. B and AAV-PHP.eB, reportedly broadly transduce cells throughout the CNS compared to the original serotype 9, AAV9. As non-human primate data are scarce, we here evaluated the CNS transduction efficiencies after lumbar intrathecal bolus delivery of identical doses of either AAV-PHP. B:CAG-EGFP or AAV-PHP. eB:CAG-EGFP in rhesus macaque monkeys. AAV-PHP.eB achieved a more efficient and widespread CNS transduction compared to AAV-PHP.B. We report a strong neuronal and oligodendroglial tropism for both variants in the putamen and in the hippocampus. This proof-of-concept experiment highlights the potential value of intrathecal infusions of AAV-PHP.eB to distribute genetic material in the CNS with cell-type specificity and introduces a new opportunity to model brain diseases in rhesus macaque monkeys and further develop gene therapies targeting the CNS in humans.
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https://www.hal.inserm.fr/inserm-03429973
Contributor : Benjamin Dehay Connect in order to contact the contributor
Submitted on : Tuesday, November 16, 2021 - 8:05:54 AM
Last modification on : Friday, April 1, 2022 - 3:50:39 AM
Long-term archiving on: : Thursday, February 17, 2022 - 6:09:22 PM

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Marie-Laure Arotcarena, Sandra Dovero, Nathalie Biendon, Nathalie Dutheil, Vincent Planche, et al.. Pilot Study Assessing the Impact of Intrathecal Administration of Variants AAV-PHP.B and AAV-PHP.eB on Brain Transduction in Adult Rhesus Macaques. Frontiers in Bioengineering and Biotechnology, Frontiers, 2021, 9, ⟨10.3389/fbioe.2021.762209⟩. ⟨inserm-03429973⟩

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