The Chloride Homeostasis of CA3 Hippocampal Neurons Is Not Altered in Fully Symptomatic Mepc2-null Mice - Archive ouverte HAL Access content directly
Journal Articles Frontiers in Cellular Neuroscience Year : 2021

The Chloride Homeostasis of CA3 Hippocampal Neurons Is Not Altered in Fully Symptomatic Mepc2-null Mice

(1) , (1) , (2) , (3) , (1) , (1) , (1) , (4) , (1)
1
2
3
4

Abstract

Rett syndrome (RTT) is an X-linked neurodevelopmental disorder caused mainly by mutations in the MECP2 gene. Mouse models of RTT show reduced expression of the cation-chloride cotransporter KCC2 and altered chloride homeostasis at presymptomatic stages. However, whether these alterations persist to late symptomatic stages has not been studied. Here we assess KCC2 and NKCC1 expressions and chloride homeostasis in the hippocampus of early [postnatal (P) day 30-35] and late (P50-60) symptomatic male Mecp2-null (Mecp2 −/y) mice. We found (i) no difference in the relative amount, but an over-phosphorylation, of KCC2 and NKCC1 between wildtype (WT) and Mecp2 −/y hippocampi and (ii) no difference in the inhibitory strength, nor reversal potential, of GABA A-receptor-mediated responses in Mecp2 −/y CA3 pyramidal neurons compared to WT at any stages studied. Altogether, these data indicate the presence of a functional chloride extrusion mechanism in Mecp2 −/y CA3 pyramidal neurons at symptomatic stages.
Fichier principal
Vignette du fichier
Belaidouni et al Front Cell Neurosci 2021.pdf (8.11 Mo) Télécharger le fichier
Origin : Publisher files allowed on an open archive

Dates and versions

inserm-03411137 , version 1 (02-11-2021)

Identifiers

Cite

Yasmine Belaïdouni, Diabe Diabira, Jinwei Zhang, Jean-Charles Graziano, Francesca Bader, et al.. The Chloride Homeostasis of CA3 Hippocampal Neurons Is Not Altered in Fully Symptomatic Mepc2-null Mice. Frontiers in Cellular Neuroscience, 2021, 15, pp.724976. ⟨10.3389/fncel.2021.724976⟩. ⟨inserm-03411137⟩
33 View
20 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More