Usefulness of Plasma SARS-CoV-2 RNA Quantification by Droplet-based Digital PCR to Monitor Treatment Against COVID-19 in a B-cell Lymphoma Patient - Archive ouverte HAL Access content directly
Journal Articles Stem Cell Reviews and Reports Year : 2021

Usefulness of Plasma SARS-CoV-2 RNA Quantification by Droplet-based Digital PCR to Monitor Treatment Against COVID-19 in a B-cell Lymphoma Patient

(1) , (2, 3) , (2) , (1) , (4) , (2, 3, 5) , (4) , (1) , (2) , (6) , (7) , (7) , (8, 1) , (9) , (3, 10) , (3) , (8, 2) , (9, 11) , (12, 13) , (10, 3)
1
2
3
4
5
6
7
8
9
10
11
12
13
Thomas Hueso
Flore Rozenberg

Abstract

We report the case of an HIV-1-infected patient, treated with anti-CD20 monoclonal antibody for a B-cell lymphoma previously treated by autologous stem cell transplant. He suffered from chronic COVID19 and we monitored by plasma SARS-CoV-2 RNA by highly sensitive droplet-based digital PCR technology (ddPCR). Under tocilizumab therapy and despite a first clinical improvement biologically associated with decreasing inflammatory markers, a slight increase of SARS-CoV-2 RNAaemia quantified by ddPCR was highlighted, confirming the absence of viral efficacy of this treatment and predicting the subsequent observed deterioration. As expected, his complete recovery, finally achieved after COVID-19 convalescent plasmatherapy, strictly paralleled plasma SARS-CoV-2 RNA clearance. With these results, we confirmed the interest of SARS-CoV-2 RNAaemia monitoring by ddPCR in COVID-19 patients, particularly during treatment, and firstly showed that this new and specific biomarker could be helpful to select eligible patient for anti-IL6 receptors therapy considering the variable levels of efficacy recently observed with such therapy.
Embargoed file
Embargoed file
Ne sera jamais visible

Dates and versions

inserm-03405587 , version 1 (27-10-2021)

Identifiers

Cite

Tali-Anne Szwebel, David Veyer, Nicolas Robillard, Deborah Eshagh, Etienne Canoui, et al.. Usefulness of Plasma SARS-CoV-2 RNA Quantification by Droplet-based Digital PCR to Monitor Treatment Against COVID-19 in a B-cell Lymphoma Patient. Stem Cell Reviews and Reports, 2021, 17 (1), pp.296-299. ⟨10.1007/s12015-020-10107-5⟩. ⟨inserm-03405587⟩
54 View
10 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More