Skip to Main content Skip to Navigation
Journal articles

Advances in multiplexed techniques for the detection and quantification of microRNAs

Abstract : MicroRNA detection is currently a crucial analytical chemistry challenge: almost 2000 papers were referenced in PubMed in 2018 and 2019 for the keywords "miRNA detection method". MicroRNAs are potential biomarkers for multiple diseases including cancers, neurodegenerative and cardiovascular diseases. Since miRNAs are stably released in bodily fluids, they are of prime interest for the development of non-invasive diagnosis methods, such as liquid biopsies. Their detection is however challenging, as high levels of sensitivity, specificity and robustness are required. The analysis also needs to be quantitative, since the aim is to detect miRNA concentration changes. Moreover, a high multiplexing capability is also of crucial importance, since the clinical potential of miRNAs probably lays in our ability to perform parallel mapping of multiple miRNA concentrations and recognize typical disease signature from this profile. A plethora of biochemical innovative detection methods have been reported recently and some of them provide new solutions to the problem of sensitive multiplex detection. In this review, we propose to analyze in particular the new developments in multiplexed approaches to miRNA detection. The main aspects of these methods (including sensitivity and specificity) will be analyzed, with a particular focus on the demonstrated multiplexing capability and potential of each of these methods.
Document type :
Journal articles
Complete list of metadata

https://www.hal.inserm.fr/inserm-03405578
Contributor : Valerie Taly Connect in order to contact the contributor
Submitted on : Wednesday, October 27, 2021 - 12:52:01 PM
Last modification on : Wednesday, November 17, 2021 - 12:34:05 PM

File

d0cs00609b.pdf
Publication funded by an institution

Identifiers

Citation

Thomas Jet, Guillaume Gines, Yannick Rondelez, Valérie Taly. Advances in multiplexed techniques for the detection and quantification of microRNAs. Chemical Society Reviews, Royal Society of Chemistry, 2021, 50 (6), pp.4141-4161. ⟨10.1039/D0CS00609B⟩. ⟨inserm-03405578⟩

Share

Metrics

Record views

28

Files downloads

35