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Correlation between magnetic resonance, X-ray imaging alterations and histological changes in an ovine model of age-related disc degeneration

N Bouhsina 1, 2, 3 C Decante 1, 2, 4 J Hardel 3 S Madec 1, 3 J Abadie 5, 3, 6 A Hamel 1, 2, 4 C Le Visage 1, 2 J Lesoeur 1, 2 J Guicheux 1, 2, 7 J Clouet 1, 8, 9 M Fusellier 1, 2, 3
1 RMeS - Regenerative Medicine and Skeleton research lab
2 Université de Nantes - UFR Odontologie
3 ONIRIS - Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique
4 Pôle hospitalo-universitaire PHU5 - FEA - Femme, Enfant, Adolescent [CHU Nantes]
5 AMaROC - Animaux modèles pour la recherche en oncologie comparée
ONIRIS - Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique
6 CRCINA-ÉQUIPE 8 - Stress Adaptation and Tumor Escape in Breast Cancer
CRCINA - Centre de Recherche en Cancérologie et Immunologie Nantes-Angers
7 Pôle hospitalo-universitaire PHU4 - OTONN - Ostéo-articulaire - Tête et cou - Odontologie - Neurochirurgie - Neurotraumatologie [CHU Nantes]
8 Université de Nantes - UFR des Sciences Pharmaceutiques et Biologiques
9 Pôle hospitalo-universitaire PHU11 - S3P - Santé Publique, Pharmacie et Prévention [CHU Nantes]
Abstract : Sheep are one of the many animal models used to investigate the pathophysiology of disc degeneration and the regenerative strategies for intervertebral disc (IVD) disease. To date, few studies have thoroughly explored ageing of ovine lumbar IVDs. Hence, the objective of the present study was to concomitantly assess the development of spontaneous age-related lumbar IVD degeneration in sheep using X-ray, magnetic resonance imaging (MRI) as well as histological analyses. 8 young ewes (< 48 months old) and 4 skeletally mature ewes (> 48 months old) were included. Disc height, Pfirrmann and modified Pfirrmann grades as well as T2-wsi and T2 times were assessed by X-ray and MRI. The modified Boos score was also determined using histology sections. Pfirrmann (2 to 3) and modified Pfirrmann (2 to 4) grades as well as Boos scores (7 to 13) gradually increased with ageing, while T2-weighted signal intensity (1.18 to 0.75), T2 relaxation time (114.36 to 70.65 ms) and disc height (4.1 to 3.2 mm) decreased significantly. All the imaging modalities strongly correlated with the histology (p < 0.0001). The present study described the suitability of sheep as a model of age-related IVD degeneration by correlation of histological tissue alterations with the changes observed using X-ray and MRI. Given the structural similarities with humans, the study demonstrated that sheep warrant being considered as a pertinent animal model to investigate IVD regenerative strategies without induction of degeneration.
Keywords : Intervertebral disc degeneration sheep magnetic resonance imaging X-ray imaging histology
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https://www.hal.inserm.fr/inserm-03367848
Contributor : Arnaud Eyraud Connect in order to contact the contributor
Submitted on : Wednesday, October 6, 2021 - 2:18:03 PM
Last modification on : Wednesday, October 20, 2021 - 3:19:38 AM

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N Bouhsina, C Decante, J Hardel, S Madec, J Abadie, et al.. Correlation between magnetic resonance, X-ray imaging alterations and histological changes in an ovine model of age-related disc degeneration. eCells and Materials Journal, AO Research Institute Davos, 2021, 42, pp.166 - 178. ⟨10.22203/ecm.v042a13⟩. ⟨inserm-03367848⟩

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