The power of imaging to understand extracellular vesicle biology in vivo
Frederik J Verweij
(1, 2)
,
Leonora Balaj
(3, 4)
,
Chantal M Boulanger
(5)
,
David R F Carter
(6, 7)
,
Ewoud B Compeer
(8)
,
Gisela D’angelo
(9)
,
Samir El Andaloussi
(7, 10)
,
Jacky G Goetz
(11)
,
Julia Christina Gross
(12)
,
Vincent Hyenne
(11, 13)
,
Eva-Maria Krämer-Albers
(14)
,
Charles P Lai
(15)
,
Xavier Loyer
(5)
,
Alex Marki
(16)
,
Stefan Momma
(17)
,
Esther Nolte-‘t Hoen
(18)
,
D. Michiel Pegtel
(19)
,
Hector Peinado
(20)
,
Graça Raposo
(9)
,
Kirsi Rilla
(21)
,
Hidetoshi Tahara
(22)
,
Clotilde Théry
(23)
,
Martin E van Royen
(24)
,
Roosmarijn Vandenbroucke
(25)
,
Ann M Wehman
(26)
,
Kenneth Witwer
(27)
,
Zhiwei Wu
(28, 29)
,
Richard Wubbolts
(18)
,
Guillaume van Niel
(1, 2)
1
IPNP - U1266 Inserm -
Institut de psychiatrie et neurosciences de Paris
2 GHU Paris Psychiatrie et Neurosciences
3 Massachusetts General Hospital [Boston]
4 HMS - Harvard Medical School [Boston]
5 PARCC (UMR_S 970/ U970) - Paris-Centre de Recherche Cardiovasculaire
6 Oxford Brookes University
7 OSP - Oxford Science Park [Oxford, UK]
8 Kennedy Institute of Rheumatology [Oxford, UK]
9 Biologie Cellulaire et Cancer
10 Karolinska Institutet [Stockholm]
11 Immuno-Rhumatologie Moléculaire
12 HMU - Health and Medical University [Potsdam, Germany]
13 FMTS - Fédération de Médecine Translationnelle de Strasbourg
14 JGU - Johannes Gutenberg - Universität Mainz = Johannes Gutenberg University
15 AS - Academia Sinica [Taipei, Taiwan]
16 La Jolla Institute for Immunology [La Jolla, CA, États-Unis]
17 Goethe-University Frankfurt am Main
18 Utrecht University [Utrecht]
19 VU - Vrije Universiteit Amsterdam [Amsterdam]
20 CNIO - Spanish National Cancer Research Center
21 IB - Institute of Biomedicine [Kuopio, Finland]
22 Hiroshima University
23 U932 - Immunité et cancer
24 Erasmus MC - Erasmus University Medical Center [Rotterdam]
25 UGENT - Universiteit Gent = Ghent University
26 University of Denver
27 JHU - Johns Hopkins University
28 NJU - Nanjing University
29 MS - Medical School [Nanjing, China]
2 GHU Paris Psychiatrie et Neurosciences
3 Massachusetts General Hospital [Boston]
4 HMS - Harvard Medical School [Boston]
5 PARCC (UMR_S 970/ U970) - Paris-Centre de Recherche Cardiovasculaire
6 Oxford Brookes University
7 OSP - Oxford Science Park [Oxford, UK]
8 Kennedy Institute of Rheumatology [Oxford, UK]
9 Biologie Cellulaire et Cancer
10 Karolinska Institutet [Stockholm]
11 Immuno-Rhumatologie Moléculaire
12 HMU - Health and Medical University [Potsdam, Germany]
13 FMTS - Fédération de Médecine Translationnelle de Strasbourg
14 JGU - Johannes Gutenberg - Universität Mainz = Johannes Gutenberg University
15 AS - Academia Sinica [Taipei, Taiwan]
16 La Jolla Institute for Immunology [La Jolla, CA, États-Unis]
17 Goethe-University Frankfurt am Main
18 Utrecht University [Utrecht]
19 VU - Vrije Universiteit Amsterdam [Amsterdam]
20 CNIO - Spanish National Cancer Research Center
21 IB - Institute of Biomedicine [Kuopio, Finland]
22 Hiroshima University
23 U932 - Immunité et cancer
24 Erasmus MC - Erasmus University Medical Center [Rotterdam]
25 UGENT - Universiteit Gent = Ghent University
26 University of Denver
27 JHU - Johns Hopkins University
28 NJU - Nanjing University
29 MS - Medical School [Nanjing, China]
Frederik J Verweij
- Function : Author
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Leonora Balaj
- Function : Author
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Ewoud B Compeer
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Gisela D’angelo
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Jacky G Goetz
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Julia Christina Gross
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Eva-Maria Krämer-Albers
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- ORCID : 0000-0001-7994-1185
Charles P Lai
- Function : Author
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Xavier Loyer
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Stefan Momma
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Hector Peinado
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Graça Raposo
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Kirsi Rilla
- Function : Author
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Hidetoshi Tahara
- Function : Author
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Clotilde Théry
- Function : Author
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- IdHAL : clotilde-thery
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- IdRef : 144451379
Roosmarijn Vandenbroucke
- Function : Author
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- ORCID : 0000-0002-8327-620X
Richard Wubbolts
- Function : Author
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- ORCID : 0000-0001-8661-7594
Guillaume van Niel
- Function : Author
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Abstract
Extracellular vesicles (EVs) are nano-sized lipid bilayer vesicles released by virtually every cell type. EVs have diverse biological activities, ranging from roles in development and homeostasis to cancer progression, which has spurred the development of EVs as disease biomarkers and drug nanovehicles. Owing to the small size of EVs, however, most studies have relied on isolation and biochemical analysis of bulk EVs separated from biofluids. Although informative, these approaches do not capture the dynamics of EV release, biodistribution, and other contributions to pathophysiology. Recent advances in live and high-resolution microscopy techniques, combined with innovative EV labeling strategies and reporter systems, provide new tools to study EVs in vivo in their physiological environment and at the single-vesicle level. Here we critically review the latest advances and challenges in EV imaging, and identify urgent, outstanding questions in our quest to unravel EV biology and therapeutic applications.
Origin : Files produced by the author(s)