Skip to Main content Skip to Navigation
Journal articles

A single amino acid substitution in the Ak molecule fortuitously provokes an alloresponse

Abstract : We discovered by chance that the R28 T cell hybridoma has dual specificity. It responds to a peptide derived from ribonuclease presented by cells displaying Ak molecules and it reacts, in the absence of added antigen, to cells expressing Ak complexes with a single amino acid substitution at position 69 of the a chain. Modelling and functional studies suggest that residue 69 is a peptide contact residue, prompting the hypothesis that R28's alloreactivity is a cross-reactive response to an unknown peptide bound in the 'groove'of the mutant Ak complex. In this report, we employ a competition assay to confirm that this alloresponse involves a groove-binding peptide, demonstrate that this peptide derives from or depends on fetal calf serum and exploit a panel of antigen-presenting cell lineseach displaying an Ak complex with a different position 69 substitutionto establish that the alloresponse is not just a heteroclitic response to ribonuclease, itself. We speculate that much of the alloreactivity against murine class I1 molecules that is revealed in v i m may prove to be directed at bovine serum-derived peptides, suggesting that in this context, alloreactivity is a misnomer.
Document type :
Journal articles
Complete list of metadata

https://www.hal.inserm.fr/inserm-03351845
Contributor : Nadine Gervois Connect in order to contact the contributor
Submitted on : Wednesday, September 22, 2021 - 4:04:01 PM
Last modification on : Tuesday, October 19, 2021 - 3:01:02 AM

File

eji.1830210131.pdf
Files produced by the author(s)

Identifiers

Collections

Citation

Paolo Dellabona, Bing-Yuan Weia, Nadine Gervois, Christophe Benoist, Diane Mathis. A single amino acid substitution in the Ak molecule fortuitously provokes an alloresponse. European Journal of Immunology, Wiley-VCH Verlag, 1991, 21 (1), pp.209-13. ⟨10.1002/eji.1830210131⟩. ⟨inserm-03351845⟩

Share

Metrics

Record views

24

Files downloads

42