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Heterogeneity of biologic responses of melanoma-specific CTL

Abstract : To better understand how Ag density influences the various biologic responses of CTL, we analyzed lysis and, at the single cell level, cytokine production by a panel of melanoma-specific CTL clones. Titration experiments done with peptide-pulsed TAP-deficient T2 cells indicated that: 1) Ag density affects both the fraction of responding cells and the amount of cytokine secreted by each cell. 2) Different responses have a relative Ag requirement that may vary between clones. Lysis and secretion of IFN-gamma, and for most clones' secretion of TNF-alpha, required lower Ag densities, by one or two logs, than IL-2 and granulocyte-macrophage CSF secretion. 3) In a significant fraction of IFN-gamma-secreting cells, IL-2 production is not induced. 4) A large fraction of cloned cells is refractory to lymphokine gene activation for about 2 wk after previous stimulation. Together these data indicate that CTL biologic responses are controlled by variable Ag thresholds and by additional parameters affecting activation requirements of each cell. A similar heterogeneity of cytokine responses was observed to Ag endogenously presented by melanoma cells. As a consequence, most melanoma lines, including those with the highest Ag expression, could trigger only low CTL fractions to secrete IL-2 and, also for most clones, granulocyte-macrophage CSF. This may be an important component of the inefficiency of specific CTL in cancer patients.
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https://www.hal.inserm.fr/inserm-03350085
Contributor : Nadine Gervois Connect in order to contact the contributor
Submitted on : Tuesday, September 21, 2021 - 10:11:02 AM
Last modification on : Tuesday, September 21, 2021 - 4:48:52 PM

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  • HAL Id : inserm-03350085, version 1
  • PUBMED : 9300706

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Jean-François Fonteneau, E Le Dréan, S Le Guiner, Nadine Gervois, E Diez, et al.. Heterogeneity of biologic responses of melanoma-specific CTL. Journal of Immunology, Publisher : Baltimore : Williams & Wilkins, c1950-. Latest Publisher : Bethesda, MD : American Association of Immunologists, 1997, 159 (6), pp.2831-9. ⟨inserm-03350085⟩

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