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Journal Articles Journal of Clinical Periodontology Year : 2021

Differences in platelet‐rich plasma composition influence bone healing

Faleh Tamimi
  • Function : Author


Aim: Platelet-rich plasma (PRP) is autologous blood-derived material that has been used to enhance bone regeneration. Clinical studies however reported inconsistent outcomes. This study aimed to assess the effect of changes in leucocyte and PRP (L-PRP) composition on bone defect healing. Materials and methods: We prepared L-PRPs using different centrifugation methods and assessed their regenerative potential in an in vivo rat model. Bilateral critical size tibial bone defects were created and filled with either: single spin L-PRP, double spin L-PRP, or filtrated L-PRP. Empty defects and defects treated with collagen scaffolds served as controls. Rats were euthanized after two weeks, and their tibias were collected and analysed using micro-CT and histology. Results: Double-spin L-PRP contained higher concentrations of platelets than single-spin L-PRP and filtrated L-PRP. Filtration of single-spin L-PRP resulted in lower concentrations of minerals and metabolites. In vivo, double-spin L-PRP improved bone healing by significantly reducing the size of bone defects (1.08±0.2 mm3 ) compared to single-spin L-PRP (1.42 ±0.27 mm3 ) or filtrated- L-PRP (1.38±0.28 mm3 ). There were fewer mast cells, lymphocytes, and macrophages in defects treated with double-spin L-PRP than in those treated with single-spin or filtrated- L-PRP. Conclusion: The preparation method of L-PRP affects their composition and potential to regenerate bone.
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Dates and versions

inserm-03344686 , version 1 (15-09-2021)



Faez Saleh Al‐hamed, Lina Abu‐nada, Rania Rodan, Stylianos Sarrigiannidis, Jose Luis Ramirez‐garcialuna, et al.. Differences in platelet‐rich plasma composition influence bone healing. Journal of Clinical Periodontology, 2021, Online ahead of print. ⟨10.1111/jcpe.13546⟩. ⟨inserm-03344686⟩
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