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Journal Articles Nature Communications Year : 2021

Small RNA mediated gradual control of lipopolysaccharide biosynthesis affects antibiotic resistance in Helicobacter pylori

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Abstract

The small, regulatory RNA RepG (Regulator of polymeric G-repeats) regulates the expression of the chemotaxis receptor TlpB in Helicobacter pylori by targeting a variable G-repeat in the tlpB mRNA leader. Here, we show that RepG additionally controls lipopolysaccharide (LPS) phase variation by also modulating the expression of a gene (hp0102) that is co-transcribed with tlpB. The hp0102 gene encodes a glycosyltransferase required for LPS O-chain biosynthesis and in vivo colonization of the mouse stomach. The G-repeat length defines a gradual (rather than ON/OFF) control of LPS biosynthesis by RepG, and leads to gradual resistance to a membrane-targeting antibiotic. Thus, RepG-mediated modulation of LPS structure might impact host immune recognition and antibiotic sensitivity, thereby helping H. pylori to adapt and persist in the host.
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Dates and versions

inserm-03340189 , version 1 (10-09-2021)

Licence

Attribution - CC BY 4.0

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Sandy R Pernitzsch, Mona Alzheimer, Belinda U Bremer, Marie Robbe-Saule, Hilde de Reuse, et al.. Small RNA mediated gradual control of lipopolysaccharide biosynthesis affects antibiotic resistance in Helicobacter pylori. Nature Communications, 2021, 12 (1), pp.4433. ⟨10.1038/s41467-021-24689-2⟩. ⟨inserm-03340189⟩
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