Full-field optical coherence tomography: novel imaging technique for extemporaneous high-resolution analysis of mucosal architecture in human gut biopsies

Lucille Quénéhervé; Raphael Olivier; Michalina Gora; Céline Bossard; Jean-François Mosnier; Emilie Benoit a La Guillaume; Claude Boccara; Charlène Brochard; Michel Neunlist; Emmanuel Coron

doi:10.1136/gutjnl-2020-321228

Journal articles

Full-field optical coherence tomography: novel imaging technique for extemporaneous high-resolution analysis of mucosal architecture in human gut biopsies

Lucille Quénéhervé ^{1, 2} Raphael Olivier ^{1, 3} Michalina Gora ⁴ Céline Bossard ⁵ Jean-François Mosnier ⁵ Emilie Benoit a La Guillaume ⁶ Claude Boccara ^{6, 7} Charlène Brochard ^{1, 8} Michel Neunlist ^{2, 1} Emmanuel Coron ^{2, 1}

1 TENS - The Enteric Nervous System in gut and brain disorders [U1235]

2 Institut des Maladies de l'Appareil Digestif

3 Service d’hépato-gastro-entérologie et assistance nutritive [CHU de Poitiers]

4 ICube - Laboratoire des sciences de l'ingénieur, de l'informatique et de l'imagerie

5 CRCINA-ÉQUIPE 3 - Anti-Tumor Immunosurveillance and Immunotherapy

CRCINA - Centre de Recherche en Cancérologie et Immunologie Nantes-Angers

6 LLTech SAS Paris

7 IL - Institut Langevin - Ondes et Images (UMR7587)

8 CHU Pontchaillou [Rennes]

Abstract : Full-field optical coherence tomography (FFOCT) is an imaging technique of biological tissue based on tissue light reflectance analysis. We evaluated the feasibility of imaging fresh digestive mucosal biopsies after a quick mounting procedure (5 min) using two distinct modalities of FFOCT. In static FFOCT mode, we gained high-resolution images of general gut tissue-specific architecture, such as oesophageal papillae, gastric pits, duodenal villi and colonic crypts. In dynamic FFOCT mode, we imaged individual epithelial cells of the mucosal lining with a cellular or subcellular resolution and identified cellular components of the lamina propria. FFOCT represents a promising dye-free imaging tool for on-site analysis of gut tissue remodelling.

Keywords : epithelial barrier gastrointesinal endoscopy histopathology

Document type :

Journal articles

Domain :

Life Sciences [q-bio] / Cancer

Complete list of metadata

https://www.hal.inserm.fr/inserm-03335659
Contributor : Elizabeth Bernardo <>
Submitted on : Monday, September 6, 2021 - 1:52:38 PM
Last modification on : Wednesday, September 15, 2021 - 3:03:24 AM

Full-field optical coherence tomography: novel imaging technique for extemporaneous high-resolution analysis of mucosal architecture in human gut biopsies

File

Restricted access

Identifiers

Collections

Citation

Export

Metrics

8

Full-field optical coherence tomography: novel imaging technique for extemporaneous high-resolution analysis of mucosal architecture in human gut biopsies

File

Restricted access

Identifiers

Collections

Citation

Export

Share

Metrics

8