Skip to Main content Skip to Navigation
Journal articles

What defines the maternal transcriptome?

Abstract : In somatic cells, RNA polymerase II (Pol II) transcription initiation starts by the binding of the general transcription factor TFIID, containing the TATA-binding protein (TBP) and 13 TBP-associated factors (TAFs), to core promoters. However, in growing oocytes active Pol II transcription is TFIID/TBP-independent, as during oocyte growth TBP is replaced by its vertebrate-specific paralog TBPL2. TBPL2 does not interact with TAFs, but stably associates with TFIIA. The maternal transcriptome is the population of mRNAs produced and stored in the cytoplasm of growing oocytes. After fertilization, maternal mRNAs are inherited by the zygote from the oocyte. As transcription becomes silent after oocyte growth, these mRNAs are the sole source for active protein translation. They will participate to complete the protein pool required for oocyte terminal differentiation, fertilization and initiation of early development, until reactivation of transcription in the embryo, called zygotic genome activation (ZGA). All these events are controlled by an important reshaping of the maternal transcriptome. This procedure combines cytoplasmic readenylation of stored transcripts, allowing their translation, and different waves of mRNA degradation by deadenylation coupled to decapping, to eliminate transcripts coding for proteins that are no longer required. The reshaping ends after ZGA with an almost total clearance of the maternal transcripts. In the past, the murine maternal transcriptome has received little attention but recent progresses have brought new insights into the regulation of maternal mRNA dynamics in the mouse. This review will address past and recent data on the mechanisms associated with maternal transcriptome dynamic in the mouse.
Document type :
Journal articles
Complete list of metadata

https://www.hal.inserm.fr/inserm-03326083
Contributor : Stéphane Vincent <>
Submitted on : Wednesday, August 25, 2021 - 3:46:08 PM
Last modification on : Friday, August 27, 2021 - 3:23:47 AM

Identifiers

Collections

Citation

László Tora, Stéphane Vincent. What defines the maternal transcriptome?. Biochemical Society Transactions, Portland Press, 2021, pp.BST20201125. ⟨10.1042/BST20201125⟩. ⟨inserm-03326083⟩

Share

Metrics

Record views

17

Files downloads

13