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Insights into the ligand binding specificity of SREC‐II (scavenger receptor expressed by endothelial cells)

Abstract : SREC-II (scavenger receptor expressed by endothelial cells-II) is a membrane protein encoded by the SCARF2 gene, with high homology to class F scavenger receptor SR-F1, but no known scavenging function. We produced the extracellular domain of SREC-II in a recombinant form and investigated its capacity to interact with common scavenger receptor ligands, including acetylated low density lipoprotein (AcLDL) and maleylated or acetylated BSA (MalBSA or AcBSA). Whereas no binding was observed for AcLDL, SREC-II ectodomain interacted strongly with MalBSA and bound with high affinity to AcBSA, a property shared with the SR-F1 ectodomain. SREC-II ectodomain also interacted with two SR-F1 specific ligands, complement C1q and calreticulin, with affinities in the 100 nM range. We proceeded to generate a stable CHO cell line overexpressing full-length SREC-II; binding of MalBSA to these cells was significantly increased compared to non-transfected CHO cells. In contrast, no increase in binding could be detected for C1q and calreticulin. We show for the first time that SREC-II has the capacity to interact with the common scavenger receptor ligand MalBSA. In addition, our data highlight similarities and differences in the ligand binding properties of SREC-II in soluble form and at the cell surface, and show that endogenous protein ligands of the ectodomain of SREC-II, such as C1q and calreticulin, are shared with the corresponding domain of SR-F1.
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https://www.hal.inserm.fr/inserm-03315913
Contributor : Elizabeth Bernardo <>
Submitted on : Friday, August 6, 2021 - 9:34:45 AM
Last modification on : Tuesday, August 31, 2021 - 3:12:41 AM

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2021DelnesteEq07.pdf
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Catherine Wicker‐planquart, Pascale Tacnet‐delorme, Laurence Preisser, Samy Dufour, Yves Delneste, et al.. Insights into the ligand binding specificity of SREC‐II (scavenger receptor expressed by endothelial cells). FEBS Open Bio, Wiley Open Access/Elsevier, 2021, Online ahead of print. ⟨10.1002/2211-5463.13260⟩. ⟨inserm-03315913⟩

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