Skip to Main content Skip to Navigation
Journal articles

An optimized protocol with a stepwise approach to identify specific nuclear receptor ligands from cultured mammalian cells

Abstract : Here, we describe an optimized protocol to identify specific nuclear receptor ligands. First, to rule out any compound interference with luciferase activity per se, we describe an in vitro assay assessing potential inhibition or activation of luciferase enzymatic activity. Second, to comply with EMA and FDA guidelines to mitigate drug-drug interactions, we detail assays assessing CAR and PXR activation ability. Finally, to minimize off-target detection effects, we describe the use of mammalian one-(or two-) hybrid systems. For complete details on the use and execution of this protocol, please refer to Hering et al. (2018).
Document type :
Journal articles
Complete list of metadata

https://www.hal.inserm.fr/inserm-03282753
Contributor : Marie-Hélène Derudas Connect in order to contact the contributor
Submitted on : Friday, July 9, 2021 - 1:58:39 PM
Last modification on : Wednesday, July 14, 2021 - 3:43:01 AM
Long-term archiving on: : Sunday, October 10, 2021 - 7:22:19 PM

File

Berthier et al 2021_star proto...
Publication funded by an institution

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Collections

Citation

Alexandre Berthier, Bart Staels, Philippe Lefebvre. An optimized protocol with a stepwise approach to identify specific nuclear receptor ligands from cultured mammalian cells. STAR Protocols, Elsevier, 2021, 2 (3), pp.100658. ⟨10.1016/j.xpro.2021.100658⟩. ⟨inserm-03282753⟩

Share

Metrics

Record views

26

Files downloads

39