Skip to Main content Skip to Navigation
Journal articles

Assessment of serological techniques for screening patients for COVID-19 (COVID-SER): a prospective, multicentric study

Abstract : INTRODUCTION: The COVID-19 pandemic caused by SARS-CoV-2 threatens global public health, and there is an urgent public health need to assess acquired immunity to SARS-CoV-2. Serological tests might provide results that can be complementary to or confirm suspected COVID-19 cases and reveal previous infection. The performance of serological assays (sensitivity and specificity) has to be evaluated before their use in the general population. The neutralisation capacity of the produced antibodies also has to be evaluated. METHODS AND ANALYSIS: We set up a prospective, multicentric clinical study to evaluate the performance of serological kits among a population of healthcare workers presenting mild symptoms suggestive of SARS-CoV-2 infection. Four hundred symptomatic healthcare workers will be included in the COVID-SER study. The values obtained from a control cohort included during the prepandemic time will be used as reference. A workflow was set up to study serological response to SARS-CoV-2 infection and to evaluate antibody neutralisation capacity in patients with a confirmed SARS-CoV-2 infection. The sensitivity and specificity of the tests will be assessed using molecular detection of the virus as a reference. The measurement of IgM and IgG antibodies will be performed once per week for 6 consecutive weeks and then at 6, 12, 18, 24 and 36 months after the diagnosis. The kinetics of IgM and IgG will determine the optimal period to perform serological testing. The proportion of false negative PCR tests in symptomatic subjects will be determined on the basis of subsequent seroconversions. ETHICS AND DISSEMINATION: Ethical approval has been obtained from the national review board for biomedical research in April 2020 (Comité de Protection des Personnes Sud Méditerranée I, Marseille, France) (ID RCB 2020-A00932-37). Results will be disseminated through presentations at scientific meetings and publications in peer-reviewed journals. TRIAL REGISTRATION NUMBER: NCT04341142.
Document type :
Journal articles
Complete list of metadata

https://www.hal.inserm.fr/inserm-03268243
Contributor : Laboratoire Carmen Connect in order to contact the contributor
Submitted on : Wednesday, June 23, 2021 - 7:56:11 AM
Last modification on : Thursday, August 5, 2021 - 10:32:33 AM
Long-term archiving on: : Friday, September 24, 2021 - 6:12:19 PM

File

e041268.full.pdf
Publication funded by an institution

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Citation

S. Trouillet-Assant, C. Albert Vega, A. Bal, J. A. Nazare, P. Fascia, et al.. Assessment of serological techniques for screening patients for COVID-19 (COVID-SER): a prospective, multicentric study. BMJ Open, BMJ Publishing Group, 2020, 10 (11), pp.e041268. ⟨10.1136/bmjopen-2020-041268⟩. ⟨inserm-03268243⟩

Share

Metrics

Record views

52

Files downloads

97