MICAL-L1 is required for cargo protein delivery to the cell surface - Archive ouverte HAL Access content directly
Journal Articles Biology Open Year : 2021

MICAL-L1 is required for cargo protein delivery to the cell surface

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Abstract

Secreted proteins are transported along intracellular route from the endoplasmic reticulum through the Golgi before reaching the plasma membrane. Small GTPase Rab and their effectors play a key role in membrane trafficking. Using confocal microscopy, we showed that MICAL-L1 was associated with tubulo-vesicular structures and exhibited a significant colocalization with markers of the Golgi apparatus and recycling endosomes. Super resolution STORM microscopy suggested at the molecular level, a very close association of MICAL-L1 and microdomains in the Golgi cisternae. Using a synchronized secretion assay, we report that the shRNAmediated depletion of MICAL-L1 impaired the delivery of a subset of cargo proteins to the cell surface. The process of membrane tubulation was monitored in vitro, and we observe that recombinant MICAL-L1-RBD domain may contribute to promote PACSINsmediated membrane tubulation. Interestingly, two hydrophobic residues at the C-terminus of MICAL-L1 appeared to be important for phosphatidic acid binding, and for association with membrane tubules. Our results reveal a new role for MICAL-L1 in cargo delivery to the plasma membrane.
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Origin : Publication funded by an institution

Dates and versions

inserm-03258482 , version 1 (11-06-2021)

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R. Sikora, P. Bun, L. Danglot, M. Alqabandi, P. Bassereau, et al.. MICAL-L1 is required for cargo protein delivery to the cell surface. Biology Open, 2021, 10 (6), pp.bio058008. ⟨10.1242/bio.058008⟩. ⟨inserm-03258482⟩
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