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[Gene amplification and chromosomal rearrangements during acquisition of cellular resistance to the antimetabolite coformycin].

Abstract : We studied the early stages of gene amplification in a Chinese hamster cell line and we show that two distinct mechanisms can operate at a single locus. Both of them rely on an unequal segregation of gene copies at mitosis. We conclude that cycles of chromatid breakage, followed by fusion of sister chromatids devoid of a telomere that lead to further breaks in mitosis, have a key role in the coupling of gene amplification and genome remodeling. Rearrangements are first limited to a single chromosome but can then potentially spread to any additional chromosome. Occasionally, a sequence containing the selected gene can be looped out, generating a "double minute" and thus initiating an independent process of extrachromosomal amplification.
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https://www.hal.inserm.fr/inserm-03199032
Contributor : Franck Toledo Connect in order to contact the contributor
Submitted on : Thursday, April 15, 2021 - 12:17:20 PM
Last modification on : Tuesday, October 19, 2021 - 10:29:27 PM

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  • HAL Id : inserm-03199032, version 1
  • PUBMED : 7749213

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Michelle Debatisse, Franck Toledo, Gérard Buttin. [Gene amplification and chromosomal rearrangements during acquisition of cellular resistance to the antimetabolite coformycin].. Bulletin du Cancer, John Libbey Eurotext, 1994, 81 (5), pp.372-80. ⟨inserm-03199032⟩

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