The Highly Conserved Chinese Hamster GNAI3 Gene Maps Less Than 60 kb from the AMPD2 Gene and Lacks the Intronic U6 snRNA Present in Its Human Counterpart - Archive ouverte HAL Access content directly
Journal Articles Genomics Year : 1994

The Highly Conserved Chinese Hamster GNAI3 Gene Maps Less Than 60 kb from the AMPD2 Gene and Lacks the Intronic U6 snRNA Present in Its Human Counterpart

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Bruno Baron
  • Function : Author
  • PersonId : 757146
  • IdRef : 163546797
Franck Toledo
  • Function : Author
  • PersonId : 980869
Nicolas Martin
Michelle Debatisse

Abstract

The identity of a gene coamplified with the adenylate deaminase 2 gene (AMPD2) in coformycin-resistant cells was determined by analysis of its genomic sequence. Sequence comparisons reveal a significant homology with the 3' terminal part of the gene encoding the alpha i3 subunit of Gi proteins from several species (GNAI3). Identification of the gene was confirmed by Western blot analysis of its products. A precise sequence comparison was performed with the human genomic sequence. It showed that conservation remains important in noncoding exons as well as in introns. However, sequences corresponding to combined U6 snRNA and E protein pseudogene, previously identified inside intron 7 of the human gene, were not found in the Chinese hamster gene. GNAI3 is mapped to a region of conserved linkage between human chromosome 1 (locus 1p13) and mouse chromosome 3 (at 48.4 cM). The Chinese hamster GNAI3 gene maps to chromosome 1 within a 120-kb fragment that also comprises the AMPD2 and GSTM genes.

Dates and versions

inserm-03199030 , version 1 (15-04-2021)

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Bruno Baron, Maria Aparecida Fernandez, Franck Toledo, Danièle Le Roscouët, Véronique Mayau, et al.. The Highly Conserved Chinese Hamster GNAI3 Gene Maps Less Than 60 kb from the AMPD2 Gene and Lacks the Intronic U6 snRNA Present in Its Human Counterpart. Genomics, 1994, 24 (2), pp.288-294. ⟨10.1006/geno.1994.1618⟩. ⟨inserm-03199030⟩
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