 |
Journal articles
Altered neuronal support and inflammatory response in bipolar disorder patient-derived astrocytes
Abstract : Bipolar disorder (BD) is characterized by cyclical mood shifts. Studies indicate that BD patients have a peripheral pro-inflammatory state and alterations in glial populations in the brain. We utilized an in vitro model to study inflammation-related phenotypes of astrocytes derived from induced pluripotent stem cells (iPSCs) generated from BD patients and healthy controls. BD astrocytes showed changes in transcriptome and induced a reduction in neuronal activity when co-cultured with neurons. IL-1b-stimulated BD astrocytes displayed a unique inflammatory gene expression signature and increased secretion of IL-6. Conditioned medium from stimulated BD astrocytes reduced neuronal activity, and this effect was partially blocked by IL-6 inactivating antibody. Our results suggest that BD astrocytes are functionally less supportive of neuronal excitability and this effect is partially mediated by IL-6. We confirmed higher IL-6 in blood in a distinct cohort of BD patients, highlighting the potential role of astrocyte-mediated inflammatory signaling in BD neuropathology.
https://www.hal.inserm.fr/inserm-03161941
Contributor : Clara Martinez Rico <>
Submitted on : Monday, March 8, 2021 - 10:52:03 AM
Last modification on : Thursday, April 15, 2021 - 3:08:12 PM
Contributor : Clara Martinez Rico <>
Submitted on : Monday, March 8, 2021 - 10:52:03 AM
Last modification on : Thursday, April 15, 2021 - 3:08:12 PM
File
1-s2.0-S2213671121000849-main....
Publication funded by an institution