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Abnormal structure and expression of p53 gene in human hepatocellular carcinoma (hepatocarcinogenesis/recessive oncogene/gene expression)

Abstract : There is little information regarding the molecular mechanisms of hepatocarcinogenesis. We studied the p53 gene at the DNA, RNA, and protein level in seven human hepatocellular carcinoma (HCC)-derived cell lines; six of seven showed p53 abnormalities. By Southern blotting, the p53 gene was found to be partially deleted in Hep 3B and rearranged in SK-HEP-1 cells. Transcripts of the p53 gene were undetectable in Hep 3B as well as in FOCUS cells that had no apparent deletion or rearrangement of the p53 gene. Immunoprecipitation after [35Sjmethionine labeling of HCC cells demonstrated that p53 protein was absent in Hep 3B and FOCUS and reduced in concentration in PLC/PRF/5 cells. p53 synthesized by
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https://www.hal.inserm.fr/inserm-03157421
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Submitted on : Wednesday, March 3, 2021 - 10:33:45 AM
Last modification on : Wednesday, March 3, 2021 - 2:24:53 PM

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Brigitte Bressac, Katherine Galvin, T. Liang, Kurt Isselbacher, Jack Wands, et al.. Abnormal structure and expression of p53 gene in human hepatocellular carcinoma (hepatocarcinogenesis/recessive oncogene/gene expression). Proceedings of the National Academy of Sciences of the United States of America , National Academy of Sciences, 1990, 87 (5), pp.1973-1977. ⟨10.1073/pnas.87.5.1973⟩. ⟨inserm-03157421⟩

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