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Journal Articles Cell Death and Disease Year : 2021

TOM20-mediated transfer of Bcl2 from ER to MAM and mitochondria upon induction of apoptosis

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Abstract

In this work, we have explored the subcellular localization of Bcl2, a major antiapoptotic protein. In U251 glioma cells, we found that Bcl2 is localized mainly in the ER and is translocated to MAM and mitochondria upon induction of apoptosis; this mitochondrial transfer was not restricted to the demonstrator cell line, even if cell-specific modulations exist. We found that the Bcl2/mitochondria interaction is controlled by TOM20, a protein that belongs to the protein import machinery of the mitochondrial outer membrane. The expression of a small domain of interaction of TOM20 with Bcl2 potentiates its anti-apoptotic properties, which suggests that the Bcl2-TOM20 interaction is proapoptotic. The role of MAM and TOM20 in Bcl2 apoptotic mitochondrial localization and function has been confirmed in a yeast model in which the ER-mitochondria encounter structure (ERMES) complex (required for MAM stability in yeast) has been disrupted. Bcl2-TOM20 interaction is thus an additional player in the control of apoptosis.
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Dates and versions

inserm-03145368 , version 1 (18-02-2021)

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Lisenn Lalier, Vincent Mignard, Marie-Pierre Joalland, Didier Lanoé, Pierre-Francois Cartron, et al.. TOM20-mediated transfer of Bcl2 from ER to MAM and mitochondria upon induction of apoptosis. Cell Death and Disease, 2021, 12 (2), pp.182. ⟨10.1038/s41419-021-03471-8⟩. ⟨inserm-03145368⟩
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