Skip to Main content Skip to Navigation
Journal articles

TgZFP2 is a novel zinc finger protein involved in coordinating mitosis and budding in Toxoplasma

Abstract : Zinc finger proteins (ZFPs) are one of the most abundant groups of proteins with a wide range of molecular functions. We have characterised a Toxoplasma protein that we named TgZFP2, as it bears a zinc finger domain conserved in eukaryotes. However, this protein has little homology outside this region and contains no other conserved domain that could hint for a particular function. We thus investigated TgZFP2 function by generating a conditional mutant. We showed that depletion of TgZFP2 leads to a drastic arrest in the parasite cell cycle, and complementation assays demonstrated the zinc finger domain is essential for TgZFP2 function. More precisely, whereas replication of the nuclear material is initially essentially unaltered, daughter cell budding is seriously impaired: to a large extent newly formed buds fail to incorporate nuclear material. TgZFP2 is found at the basal complex in extracellular parasites and after invasion, but as the parasites progress into cell division, it relocalises to cytoplasmic punctate structures and, strikingly, accumulates in the pericentrosomal area at the onset of daughter cell elongation. Centrosomes have emerged as major coordinators of the budding and nuclear cycles in Toxoplasma, and our study identifies a novel and important component of this machinery.
Document type :
Journal articles
Complete list of metadata
Contributor : Sébastien Besteiro Connect in order to contact the contributor
Submitted on : Tuesday, February 16, 2021 - 5:15:39 PM
Last modification on : Friday, August 5, 2022 - 10:58:04 AM
Long-term archiving on: : Monday, May 17, 2021 - 9:08:29 PM


 Restricted access
To satisfy the distribution rights of the publisher, the document is embargoed until : jamais

Please log in to resquest access to the document




Ksenia Semenovskaya, Maude Lévêque, Laurence Berry, Yann Bordat, Jean-Francois Dubremetz, et al.. TgZFP2 is a novel zinc finger protein involved in coordinating mitosis and budding in Toxoplasma. Cellular Microbiology, Wiley, 2019, 22 (1), pp.e13120. ⟨10.1111/cmi.13120⟩. ⟨inserm-03143284⟩



Record views