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Phenotypic differences of CD4 + T cells in response to red blood cell immunization in transfused sickle cell disease patients

Abstract : Alloimmunization against red blood cells (RBCs) is the main immunological risk associated with transfusion in patients with sickle cell disease (SCD). However, about 50-70% of SCD patients never get immunized despite frequent transfusion. In murine models, CD4(+) T cells play a key role in RBC alloimmunization. We therefore explored and compared the CD4(+) T-cell phenotypes and functions between a group of SCD patients (n = 11) who never became immunized despite a high transfusion regimen and a group of SCD patients (n = 10) who had become immunized (at least against Kidd antigen b) after a low transfusion regimen. We studied markers of CD4(+) T-cell function, including TLR, that directly control lymphocyte function, and their spontaneous cytokine production. We also tested responders for the cytokine profile in response to Kidd antigen b peptides. Low TLR2/TLR3 expression and, unexpectedly, strong expression of CD40 on CD4(+) T cells were associated with the nonresponder status, whereas spontaneous expression of IL-10 by CD4(+) T cells and weak Tbet expression were associated with the responder status. A Th17 profile was predominant in responders when stimulated by Jb(k) . These findings implicate CD4(+) T cells in alloimmunization in humans and suggest that they may be exploited to differentiate responders from nonresponders.
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Contributor : Benoit Vingert Connect in order to contact the contributor
Submitted on : Wednesday, February 3, 2021 - 9:43:28 AM
Last modification on : Tuesday, October 19, 2021 - 4:10:51 PM
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Benoît Vingert, Marie Tamagne, Anoosha Habibi, Sadaf Pakdaman, Julie Ripa, et al.. Phenotypic differences of CD4 + T cells in response to red blood cell immunization in transfused sickle cell disease patients. European Journal of Immunology, Wiley-VCH Verlag, 2015, 45 (6), pp.1868-1879. ⟨10.1002/eji.201445187⟩. ⟨inserm-03129784⟩



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