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Anti-CD20 Antibody Prevents Red Blood Cell Alloimmunization in a Mouse Model

Abstract : Alloimmunization against RBCs can cause life-threatening delayed hemolytic transfusion reactions. Anti-CD20 Ab has recently been used to prevent alloimmunization. However, its effects remain unclear, particularly in lymphoid organs. We investigated the impact of murine anti-CD20 Ab in the blood and spleen. We assessed protocols for preventing primary alloimmunization and for abolishing established alloimmunization. Prophylactic protocols prevented alloimmunization. However, anti-CD20 treatment could only limit the further amplification of established alloimmunization. Residual B cell subtype distribution was disrupted in the spleen, but adoptive transfer studies indicated that these cells were neither plasma nor memory cells. Anti-CD20 Ab had a major effect on alloreactive CD4+ T cells, increasing the expansion of this population and its CD40 expression, while lowering its CD134 expression, thereby confirming its role in alloimmunization. In conclusion, this study shows that anti-CD20 immunotherapy can prevent RBC Ab development. However, this immunotherapy is limited by the increase in alloreactive CD4+ T lymphocytes. Nevertheless, treatment with anti-CD20 Abs should be considered for patients requiring transfusion with a very high risk of alloimmunization and life-threatening complications.
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https://www.hal.inserm.fr/inserm-03129773
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Submitted on : Wednesday, February 3, 2021 - 9:40:15 AM
Last modification on : Wednesday, November 3, 2021 - 6:48:43 AM
Long-term archiving on: : Tuesday, May 4, 2021 - 6:22:20 PM

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Rahma Elayeb, Marie Tamagne, Marion Pinheiro, Julie Ripa, Rachid Djoudi, et al.. Anti-CD20 Antibody Prevents Red Blood Cell Alloimmunization in a Mouse Model. Journal of Immunology, Publisher : Baltimore : Williams & Wilkins, c1950-. Latest Publisher : Bethesda, MD : American Association of Immunologists, 2017, 199, pp.3771 - 3780. ⟨10.4049/jimmunol.1700754⟩. ⟨inserm-03129773⟩

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